FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_11_NxW_C4-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_11_NxW_C4-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69556325
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT82200.011.4132481
CGGGGAT117450.011.3558131
CGGGAAT193950.09.4938481
GCGGGAT128550.09.4221561
GCCGAAT99050.09.4009881
GCCGGAT165700.09.2110741
CGGGGAG95350.08.8479491
CCGGCAT121050.08.7045831
CCGGGAT181800.08.6264161
GCGGAAT142350.08.3857751
GTCGGAT260900.08.3591151
GCCAGAT223750.08.3076421
CGGGCCC20650.08.30658151
CGCGGAT108000.08.2329651
GGGAGAT385550.08.1897771
CCCGTAT72850.08.168941
CGGGTAT103050.08.0169711
GTCGAAT255100.08.01398851
CGCGGGG45000.08.0125551
CGCCCAT105650.07.98534771
Produced by FastQC (version 0.11.5)