FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_10_NxW_C4-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_10_NxW_C4-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46240189
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT63100.012.2048661
CGGGGAT81450.011.6900821
CGGGAAT129450.09.7350941
CGCGTAT36200.09.5734031
GCGGGAT106950.09.5574451
CCGGCAT78200.09.53482151
CGGGCGC24700.09.49385115
CCGGGAT117350.09.3368631
GCCGGAT107100.09.1518371
GGGGGAT78200.08.9081191
CGCGAAT63300.08.6823181
GCCGAAT69850.08.619891
CGCGGAT71700.08.2510131
CGCGGGG23700.08.1237031
GGGGAAT125050.08.1180931
GGGAGAT250250.08.0992381
CGCCCTT48250.08.0531431
GCGGGTT97200.07.99514681
GCGGAAT96650.07.96820551
GCCAGAT137350.07.84985731
Produced by FastQC (version 0.11.5)