FastQCFastQC Report
Fri 10 Nov 2023
HJM7CAFX5_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJM7CAFX5_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3357175
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGCATGCTCCACAAACAGCTTGCACATTCCTTTCTCACAGCGTGTGC3176199.460900906267918No Hit
CATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACC2220736.614877091602314No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG353931.0542494805900795No Hit
GTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCG326340.9720672887174484No Hit
GACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGAC270420.8054986707574076No Hit
CCGTACCTCATTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA251740.7498566503086673No Hit
TTCCTGCCATTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA204270.6084580041254924No Hit
TTCCATCCATTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA197970.5896922263510243No Hit
CCGTACCTCATTGTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA155270.4625019547685182No Hit
CCGTACCTCATTGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA151210.45040845353608316No Hit
TTCAATCCATTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA147820.4403106778764885No Hit
TTCCATCCATTACTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140480.41844705742179067No Hit
TTCCTGCCATTACTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA136480.4065322778824458No Hit
GTGCAACCAGTTGCGTCAAAGACTTGGACTGGTGTGAGAATAGTTGGCCA131390.39137072091862946No Hit
CCCTAGAGCAGCGAAAGCATAACAGGAGGTCTGCTGTCTCTTATACACAT120980.3603625071674846No Hit
CAATAGGGCGCCCTGATTTGCAGATTTACAACCGGAATGGGAACCAGGGC119450.35580510399368515No Hit
TTCCTGCCATTACTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA117540.350115796763648No Hit
CAGACACTCATTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA111560.33230320135232744No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN109520.3262266637872616No Hit
TTCCATCCATTACTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA109510.32619687683841325No Hit
CCTATGTGCTGTTAACCCTCCATGTTCACCTAGCTTCTGTCTCTTATACA108780.32402242957248284No Hit
CCCCCGGCCGGGCCCTGAGTGACGCCCCAGGCCAGAAATCTGGGCCTTTC94060.2801760408676938No Hit
TTCAATCCATTACTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA93510.2785377586810339No Hit
TTCAATCCATTACTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA77450.23069991883056437No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT73350.21848726980273595No Hit
CAGACACTCATTGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71540.2130958320611824No Hit
CAGACACTCATTGTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64370.19173858973690677No Hit
GGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATG53880.16049208039497492No Hit
CAGACACTCATTGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47220.1406539724619658No Hit
CAGAAACTCATTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41950.12495625041887898No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA41610.12394349415803467Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCCATCCATTACAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40090.11941587793308363No Hit
TTCCATCCATTACAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40030.11923715623999344No Hit
CAAGGCATGCTCCACAACCAGCTTGCACATTCCTTTCTCACAGCGTGTGC38700.11527549204316129No Hit
CAGAAACTAATTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36570.10893087193846016No Hit
CAGACACTAATTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35490.10571388146283706No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGAGAGATGGCTCAG35070.10446282961120584Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGACACTCATTGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34840.10377772978769353No Hit
CATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGCAGCCATACC34020.10133519998212782No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)