FastQCFastQC Report
Fri 10 Nov 2023
HJM7CAFX5_n02_liv_RNA_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJM7CAFX5_n02_liv_RNA_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30811591
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGAGAGATGGCTCAG1163200.37752026501974534Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA1095170.3554409118308756Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1082470.35131908637888903Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTCAGGCAGCGAGAG811040.2632256153211952Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTCTTAGCGGTGGATCAC678340.22015740764571357Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCCTGCCATTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA601740.19529663365971592No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA594150.19283327498407984No Hit
CCGTACCTCATTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA537010.1742883059819923No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG420030.13632207437778854No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGTAGTGGTGGCGCACG403160.13084686214353553Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCCTGCCATTACTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA392930.12752668305898257No Hit
TTCCTGCCATTACTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA375210.12177560061731314No Hit
CCGTACCTCATTGTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA324100.10518768732195621No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAACAAGCGAGATAGGA319240.10361035884190466Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)