FastQCFastQC Report
Fri 10 Nov 2023
HJM7CAFX5_n02_liv_RNA_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJM7CAFX5_n02_liv_RNA_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5238255
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGAGAGATGGCTCAG189250.36128443537017574Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA188070.35903177680353476Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA180500.34458039938872775Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTCAGGCAGCGAGAG138720.2648210138681679Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTCTTAGCGGTGGATCAC117740.22476950816636454Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA105550.20149839975335299No Hit
TTCCTGCCATTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA104190.19890211530366508No Hit
CCGTACCTCATTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA88740.16940756034213683No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGTAGTGGTGGCGCACG71600.13668674014533466Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG69460.13260141020244337No Hit
TTCCTGCCATTACTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA69190.13208597137787298No Hit
TTCCTGCCATTACTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68680.13111236470924No Hit
CCGTACCTCATTGTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58660.11198385721962753No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAACAAGCGAGATAGGA57630.11001755355552564Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAACAAGCGAGATAGGAA53110.10138872582568051Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAG52920.10102600961579764Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)