FastQCFastQC Report
Fri 10 Nov 2023
HJM7CAFX5_n02_liv_RNA_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJM7CAFX5_n02_liv_RNA_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35886425
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGAGAGATGGCTCAG1328160.37010095042902713Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA1273270.3548054731002043Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1234830.34409390180270116Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTCAGGCAGCGAGAG943050.2627873910538595Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTCTTAGCGGTGGATCAC786550.21917758595346293Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA701040.1953496342976488No Hit
TTCCTGCCATTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA671030.18698714068063343No Hit
CCGTACCTCATTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA606620.16903884964857882No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG494060.13767322880448526No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGTAGTGGTGGCGCACG481560.13419001753448553Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCCTGCCATTACTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA437060.1217897854132865No Hit
TTCCTGCCATTACTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA432900.12063057270263058No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAACAAGCGAGATAGGA395400.11018093889263142Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCGTACCTCATTGTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA382030.1064552961182397No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAACAAGCGAGATAGGAA365090.10173484820513606Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAG359510.10017994269420818Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)