FastQCFastQC Report
Fri 10 Nov 2023
HJM7CAFX5_n02_liv_Hash_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJM7CAFX5_n02_liv_Hash_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6010141
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGTACCTCATTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66450511.056396181054653No Hit
TTCCTGCCATTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4999858.319022798300406No Hit
CCGTACCTCATTGTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3700476.157043570192446No Hit
CCGTACCTCATTGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3662526.093900292855026No Hit
TTCCTGCCATTACTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3193985.314317917000616No Hit
TTCCTGCCATTACTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2713154.514286769644839No Hit
CCGTACCTCATTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT724281.2050965193661844No Hit
TTCCTGCCATTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT573600.9543869270288335No Hit
CCGTACCTCATTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT463950.771945283812809No Hit
CCGTACCTCATTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC423770.7050916109954825No Hit
CCGTACCTCATTGTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT414850.690250029075857No Hit
CCGTACCTCATTGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT402470.669651510671713No Hit
TTCCTGCCATTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT375780.6252432347261071No Hit
TTCCTGCCATTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC365520.6081720878095872No Hit
TTCCTGCCATTACTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT361140.600884405207798No Hit
TTCCTGCCATTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG337730.5619335719411575No Hit
TTCCTGCCATTACTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT323180.5377244893256248No Hit
CCGTACCTCATTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG317070.5275583384815764No Hit
CCGTACCTCATTGTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT277380.461519954357144No Hit
CCGTACCTCATTGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC273150.45448184992664903No Hit
CCGTACCTCATTGTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC271050.4509877555285309No Hit
CCGTACCTCATTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA257620.4286421899253279No Hit
CCGTACCTCATTGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT256230.4263294322046687No Hit
CCGTACCTCATTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA244330.4065295639486661No Hit
TTCCTGCCATTACTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC240290.399807591868477No Hit
TTCCTGCCATTACTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT238900.39749483414781783No Hit
TTCCTGCCATTACTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT220340.3666136950863549No Hit
TTCCTGCCATTACTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG217850.36247069744287197No Hit
CCGTACCTCATTGTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG213560.35533276174385925No Hit
TTCCTGCCATTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA209990.34939280126705846No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG209390.3483944885818819No Hit
TTCCTGCCATTACTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC207350.34500022545228143No Hit
CCGTACCTCATTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT207290.3449003941837637No Hit
TTCCTGCCATTACTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG205970.3427041062763752No Hit
CCGTACCTCATTGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG199380.3317393052841855No Hit
TTCCTGCCATTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA198990.33109040203882073No Hit
CCGTACCTCATTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC183340.3050510795004643No Hit
TTCCTGCCATTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG182450.30357024901745233No Hit
CCGTACCTCATTGTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA177910.29601634969961604No Hit
CCGTACCTCATTGTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA175010.29119117172126247No Hit
CCGTACCTCATTGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA170750.28410315165650857No Hit
TTCCTGCCATTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT169720.28238938154695536No Hit
TTCCTGCCATTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC166720.2773978181210724No Hit
CCGTACCTCATTGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA157530.2621069954931174No Hit
CCGTACCTCATTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG154340.25679929971692844No Hit
CCGTACCTCATTGTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT149210.2482637262586685No Hit
TTCCTGCCATTACTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA149080.24804742517688022No Hit
CCGTACCTCATTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA144260.2400276466059615No Hit
TTCCTGCCATTACTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA138970.23122585643165441No Hit
TTCCTGCCATTACTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA138620.23064350736530143No Hit
TTCCTGCCATTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGA137000.2279480631153246No Hit
CCGTACCTCATTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAA130170.21658393704906423No Hit
CCGTACCTCATTGTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC129440.21536932328209937No Hit
TTCCTGCCATTACTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA128040.2130399270166873No Hit
CCGTACCTCATTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGA127900.21280698739014609No Hit
CCGTACCTCATTGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT127670.2124243008608284No Hit
CCGTACCTCATTGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC125960.20957910970807506No Hit
TTCCTGCCATTACTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT124300.20681711127908645No Hit
TTCCTGCCATTACTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT123370.20526972661706272No Hit
TTCCTGCCATTACTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG119980.1996292599458149No Hit
TTCCTGCCATTACTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG117040.19473752778844955No Hit
CCGTACCTCATTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAA116450.1937558536480259No Hit
CCGTACCTCATTGTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAA112950.1879323629844957No Hit
CCGTACCTCATTGTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG112530.18723354410487208No Hit
TTCCTGCCATTACTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC111270.18513708746600122No Hit
TTCCTGCCATTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAA110520.18388919660953046No Hit
CCGTACCTCATTGTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGA104290.1735233832284467No Hit
CCGTACCTCATTGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAA103670.17249179345376423No Hit
TTCCTGCCATTACTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC101180.16834879581028134No Hit
CCGTACCTCATTGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG100580.1673504831251047No Hit
CCGTACCTCATTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTA96880.16119422156651567No Hit
TTCCTGCCATTACTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGA94540.15730080209432692No Hit
TTCCTGCCATTACTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGA94270.15685156138599743No Hit
TTCCTGCCATTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAA91050.15149394997554966No Hit
CCGTACCTCATTGTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAA90290.15022942057432595No Hit
CCGTACCTCATTGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGA89870.14953060169470234No Hit
TTCCTGCCATTACTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAA88540.14731767524256087No Hit
TTCCTGCCATTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATG87440.1454874353197371No Hit
TTCCTGCCATTACTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAA86190.14340761722561915No Hit
CCGTACCTCATTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATG84530.14064561879663057No Hit
TTCCTGCCATTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA79450.13219323806213532No Hit
CCGTACCTCATTGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAA79200.13177727444331172No Hit
TTCCTGCCATTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTA76640.1275178069865582No Hit
TTCCTGCCATTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGG75970.12640302448811103No Hit
CCGTACCTCATTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCC71600.11913198043107474No Hit
CCGTACCTCATTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTTT70730.11768442703756866No Hit
TTCCTGCCATTACTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAA69840.11620359655455671No Hit
CCGTACCTCATTGTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTA69180.11510545260086244No Hit
TTCCTGCCATTACTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAA68690.11429016390796821No Hit
CCGTACCTCATTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAA66600.11081270805460305No Hit
TTCCTGCCATTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCC65190.10846667324443802No Hit
TTCCTGCCATTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAA63350.10540518100989643No Hit
CCGTACCTCATTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCA63050.10490602466730814No Hit
TTCCTGCCATTACTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGG62710.10434031414570807No Hit
CCGTACCTCATTGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTA62070.10327544728151968No Hit
CCGTACCTCATTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGG61550.10241024295436663No Hit
CCGTACCTCATTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAA61080.10162823135097829No Hit
CCGTACCTCATTGTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCC60660.10092941247135466No Hit

[OK]Adapter Content

Adapter graph