FastQCFastQC Report
Fri 10 Nov 2023
HJM7CAFX5_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJM7CAFX5_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3357175
Sequences flagged as poor quality0
Sequence length151
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAA1499254.465808306090686No Hit
CCTCCATCCCAGATAGCCCTGTGCCCTCTGGAATCCACAGGCTGGCCCCT1031253.0717790999873404No Hit
CCTCCATCCCAGATAGCCCTGTGCCCTCTGGAATCCACAGGCAGGCCCCT181370.5402458912627432No Hit
GCATAACAGGAGGTCTGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC132800.3955706807062485No Hit
CCTCCATCCCAGATAGCCCTGTGCCCTCTGGAATCCACAGTCTGGCCCCT130090.3874984175683424No Hit
GTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGAT115170.3430562898865862No Hit
TGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCATCAA109750.32691176361077395No Hit
CCTCCATCCCAGATAGCCCTGTGCCCTCTGGAATCCACCGGCTGGCCCCT106940.3185416309843842No Hit
CCTCCATCCCAGATAGCCCTGTGCCCTCTGGAATCCACACGCTGGCCCCT106460.3171118574396628No Hit
TGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGAATTATCAGCAA105290.31362678442440445No Hit
CCTCCATCCCAGATAGCCCTGTGCCCTCTGGAAACCACAGGCTGGCCCCT99890.2975418320462889No Hit
CCTCCATCCCAGATAGCCCTGTGCCCTCTGGAATCCACAGGCTCGCCCCT91400.2722527124740295No Hit
TGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGCTTTATCAGCAA88030.2622145107121315No Hit
AAGCTAGGTGAACATGGAGGGTTAACAGCACATAGGCTGTCTCTTATACA85400.25438054316501224No Hit
GGTTGCATGGCCACTAGATTCTATACCGTCATGAGACTTTATACGCTCCC84000.25021037032624155No Hit
CCTCCATCCCAGATAGCCCTGTGCCCTCTGGAATCCCCAGGCTGGCCCCT83750.24946569660503248No Hit
CAGACCTCCTGTTATGCTTTCGCTGCTCTAGGGCTGTCTCTTATACACAT74660.22238936010187138No Hit
TTTCTGCGTCAACTCCAGTTGCCTCTAAGTCTAATCCGACGTTGGAACAG66580.1983215054323948No Hit
CCTCCATCCCAGATAGCCCTGTGCCCTCTGGAATCCACACGCAGGCCCCT65370.19471728462174298No Hit
GCATAACAGGAGGTCTGAATTCACTGGCCGTCGTTTTACAACCTCGTGAC54080.1610878193719422No Hit
TGCAATGATACCGCGAGACCCACGCTCACCGGCTCCCGATTTATCAGCAA52050.15504106875572468No Hit
CCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGT51910.1546240514718476No Hit
TGCAATGATACCGCGAGACCCACGCTCACCTGCTCCAGATTTATCAGCAA50670.15093046981465072No Hit
TGCAATGATACCGCGAGACCCACGCTCACCGGCACCAGATTTATCAGCAA48830.14544967122655209No Hit
TGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTCTCAGCAA46000.1370199647024656No Hit
CCTCCATCCCAGATAGCCCTGTGCCCTCTGGAATCCACAGGCACGCCCCT44220.13171788780745716No Hit
ATCCCAAAGGGGCCGCCGGTGTCCTCTGACCACGAAAGGCCCAGATTTCT42580.1268328281963258No Hit
CCTCCATCCCAGATAGCCCTGTGCCCTCTGGAATCCACAGGCTGTCCCCT41380.12325839433452233No Hit
CCTCCATCCCAGATAGCCCTGTGCCCTCTGTAATCCACAGGCTGGCCCCT37790.11256487969796033No Hit
CCTCCATCCCAGATAGCCCTGTGCCCTCTGGAATCCACACGCACGCCCCT35600.10604153790016904No Hit
CCTCCATCCCAGATAGCCCTGTGCCCTCTGGAATCCACACGCTCGCCCCT35510.10577345536053379No Hit
CCTCCATCCCAGATAGCCCTGTGCCCTCTGGAAACCACAGGCAGGCCCCT33640.10020329592589007No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)