FastQCFastQC Report
Fri 8 Apr 2022
HJLKNBGXL_n02_Optix_Lib2_March_31_2022.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJLKNBGXL_n02_Optix_Lib2_March_31_2022.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111346188
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1613450.14490392791893333No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCA1441990.1295051070809896Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)