FastQCFastQC Report
Sat 12 Mar 2022
HJLH2AFX3_n02_dad2_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJLH2AFX3_n02_dad2_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7461665
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT330100.4423945593912351Illumina Single End PCR Primer 1 (100% over 31bp)
TCGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG315980.42347116896832004Illumina Single End PCR Primer 1 (100% over 32bp)
CGTCGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG234980.31491630889352445Illumina Single End PCR Primer 1 (100% over 30bp)
CGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGA228130.3057360522081868Illumina Single End PCR Primer 1 (100% over 29bp)
CGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG148190.19860178659856748Illumina Single End PCR Primer 1 (100% over 32bp)
CGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT147480.19765025634359087Illumina Single End PCR Primer 1 (100% over 33bp)
TCGTGTAGGGAAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA139780.18733084371919673Illumina Single End PCR Primer 1 (100% over 34bp)
CGTCGTGTAGGGAAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG121640.16301991579627334Illumina Single End PCR Primer 1 (100% over 32bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC102340.13715437506240227Illumina Single End PCR Primer 1 (100% over 50bp)
TCGGAAGAGCGTCGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTA76740.10284567854493601Illumina Single End PCR Primer 1 (96% over 29bp)
TCGTGTAGGGAAAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA75970.10181373728249661Illumina Single End PCR Primer 1 (100% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)