FastQCFastQC Report
Sat 12 Mar 2022
HJLH2AFX3_n01_dad2_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJLH2AFX3_n01_dad2_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7461665
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGT354900.4756311091425305Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC329450.4415234401437213Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCA260910.3496672659520362Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAG255880.34292614315973713Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACACTCTTTCCCTACACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC158990.21307576794187358Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CACTCTTTCCCTACACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA153630.20589238460852907Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC145800.19539874813463218Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC131790.17662277789206565Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CACTCTTTCCCTACACGACGCTAGATCGGAAGAGCACACGTCTGAACTCC96290.12904626514323544Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCT94020.12600404869422577Illumina Single End PCR Primer 1 (96% over 29bp)
ACACTCTTTCCCTACACGACGCTAGATCGGAAGAGCACACGTCTGAACTC92960.12458345422904941Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG79830.10698684542927081TruSeq Adapter, Index 2 (97% over 35bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTC77230.10350236843921563Illumina Single End PCR Primer 1 (96% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTA76870.10301990239443878TruSeq Adapter, Index 14 (97% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)