FastQCFastQC Report
Fri 8 Apr 2022
HJLCMBGXL_n01_AKM333.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJLCMBGXL_n01_AKM333.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20407605
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC6629793.2486859678046494TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC1154950.5659409813155438TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATTC379770.1860923905573437TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGG287440.14084945293678508TruSeq Adapter, Index 2 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTTTGC280800.13759576393212236TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGGATGC277370.1359150179553162TruSeq Adapter, Index 2 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)