FastQCFastQC Report
Thu 22 Sep 2016
HJKYNBCXX_l02n01_vs9_160909.3510000006898e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKYNBCXX_l02n01_vs9_160909.3510000006898e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7998603
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG570590.7133620708516224TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG65700.041.02509311
ACGTCTG67000.040.22908415
CACACGT67700.040.01252412
ACACGTC67500.039.93109513
GATCGGA69450.039.2799071
CACGTCT68600.039.1596114
CGTCTGA69200.039.01515216
AGCACAC69600.038.92022710
GAGCACA71500.037.8859869
CAGTCAC71000.037.61761527
TCCAGTC71600.037.14571825
GAACTCC72350.036.97789821
AGTCACA73000.036.7712428
CCAGTCA73050.036.62355426
AGAGCAC74500.036.4509778
CTCGTAT73400.036.38624244
TCGGAAG75550.036.1528363
GTCACAT74650.036.01831429
TCTCGTA74400.035.98790743
CACATGT75400.035.71949831
Produced by FastQC (version 0.11.2)