FastQCFastQC Report
Thu 22 Sep 2016
HJKYNBCXX_l02n01_vs5_160909.35100000068af7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKYNBCXX_l02n01_vs5_160909.35100000068af7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5760876
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC106200.18434696389924032TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG14900.033.6726911
ACACGTC15650.032.49027313
CACACGT15900.031.83791512
ACGTCTG17150.029.38617915
CGTCTGA17400.029.35187716
GATCGGA17300.029.2738881
CACGTCT17200.029.0391414
CATCTCG17650.028.17398339
AGCACAC18200.027.81444210
CGTATGC18350.027.58875344
GAACTCC19250.026.53311521
GTATGCC18900.026.42875945
TCCAGTC19400.026.2119825
CAGTCAC19700.025.4701727
CTCGTAT20000.024.86246142
CCAGTCA20850.024.71283526
GAGCACA20750.024.6131389
AGTCACC21450.023.60156828
TCGTATG21650.023.17568643
TCGGAAG22050.023.1620243
Produced by FastQC (version 0.11.2)