FastQCFastQC Report
Thu 22 Sep 2016
HJKYNBCXX_l02n01_vs24_160909.35100000068add.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKYNBCXX_l02n01_vs24_160909.35100000068add.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7746982
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG12850.026.43789511
ACACGTC14000.025.06972513
GATCGGA14650.023.3538281
ACGTCTG15200.022.79450415
CACGTCT15850.022.56944714
CGTCTGA15450.022.2800416
CACACGT16250.021.3216312
AGCACAC17850.019.5364910
CAGTCAC17600.019.0489727
GAGCACA19450.018.3920699
AGAGCAC19800.018.0669568
CTCGTAT19400.017.97651344
TATCTCG18800.017.95183841
TCCAGTC19650.017.40553325
TCGGAAG20900.017.0084153
CCAGTCA20400.016.98589726
TCGTATG21900.016.64356645
ATCTCGT21000.016.49962642
GTCACAT20900.016.47166429
TCTCGTA22300.016.14323643
Produced by FastQC (version 0.11.2)