FastQCFastQC Report
Thu 22 Sep 2016
HJKYNBCXX_l02n01_vs23_160909.35100000068ab3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKYNBCXX_l02n01_vs23_160909.35100000068ab3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10669158
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATG149390.140020421480308TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG23650.032.0595411
GATCGGA26450.028.6795541
CACGTCT26700.028.22877514
CACACGT27950.027.3687912
ACACGTC27900.027.33719613
ACGTCTG27700.027.12846215
GTCACAC29450.025.74693329
CGTCTGA30050.024.85719516
AGCACAC30900.024.6102910
TATCTCG30950.024.20819941
CAGTCAC31750.024.02398127
GAGCACA31850.023.876239
CACACTG32650.023.49914631
CCAGTCA32850.023.15103126
TCCAGTC33650.022.53419325
AGAGCAC34450.022.3354848
GAACTCC36150.021.100121
AGTCACA36750.021.0001228
TCGGAAG36400.020.953513
ATCTCGT36450.020.80229442
Produced by FastQC (version 0.11.2)