FastQCFastQC Report
Thu 22 Sep 2016
HJKYNBCXX_l02n01_vs22_160909.35100000068a9a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKYNBCXX_l02n01_vs22_160909.35100000068a9a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10101636
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATG106990.10591353717358258TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG18700.029.23533611
CACGAGT18850.028.76655231
AGTCACG21050.026.18840428
GTCACGA21400.026.07487129
ACGAGTG21000.026.03646532
CGAGTGG20950.025.776433
ACACGTC21850.025.43249513
GATCGGA22000.025.2698481
CACACGT22100.025.14479612
ACGTCTG22750.024.52526515
TCACGAG22700.024.1851230
CACGTCT24350.022.7289614
AGCACAC24550.022.26887110
CGTCTGA25650.021.40158316
TATCTCG25950.021.24275641
CAGTCAC27000.020.33422327
GAGCACA27700.019.980159
TCGGAAG28850.019.651613
TCCAGTC28950.019.35327325
AGAGCAC29450.019.1748458
Produced by FastQC (version 0.11.2)