FastQCFastQC Report
Thu 22 Sep 2016
HJKYNBCXX_l02n01_vs21_160909.35100000068a8d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKYNBCXX_l02n01_vs21_160909.35100000068a8d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18041499
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG20850.019.85611233
GCACACG21900.019.51919411
CGTACGT25300.016.63135334
GATCGGA26200.016.151661
CACACGT26900.015.72381512
ACACGTC26900.015.556540513
CACGTCT27350.015.21832314
ACGTCTG28400.014.57645415
CGTCTGA30500.014.08918616
AGCACAC33500.012.82746710
ACCGTAC33800.012.58142932
TCCAGTC36750.011.63339325
GAGCACA36250.011.6060919
GTCACCG36950.011.5087329
CAGTCAC35850.011.48570427
GTACGTA37150.011.38690135
CCAGTCA37300.011.34082726
GAACTCC40450.010.84735421
AGTCACC39450.010.779675528
AGAGCAC39350.010.7489378
Produced by FastQC (version 0.11.2)