FastQCFastQC Report
Thu 22 Sep 2016
HJKYNBCXX_l02n01_vs20_160909.35100000068a63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKYNBCXX_l02n01_vs20_160909.35100000068a63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9364911
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG10250.020.41322511
CACACGT11950.017.5092512
ACGTCTG11700.017.30649615
GATCGGA11700.016.929321
AGTCACG12350.016.57930828
ACACGTC12900.016.56862313
CGTCTGA13750.015.21713116
CACGTCT14400.014.06152814
CGTTTCG15250.013.86900633
GTCACGT14700.013.622629
AGCACAC15550.013.31097710
GTTTCGG16850.012.41933134
GAGCACA17350.012.189369
CAGTCAC18600.011.25042427
CGGAATC18550.011.1596338
GAACTCC19050.010.98478321
TCCAGTC20100.010.74678925
AGAGCAC19100.010.4835698
CCAGTCA20150.010.38500826
TCTCGTA19150.010.33948443
Produced by FastQC (version 0.11.2)