FastQCFastQC Report
Thu 22 Sep 2016
HJKYNBCXX_l02n01_vs1_160909.35100000068964.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKYNBCXX_l02n01_vs1_160909.35100000068964.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10535485
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC649480.6164690092577608TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG73050.040.77771811
GATCGGA75000.039.88681
ACACGTC75700.039.3205113
CACACGT76700.038.83719312
ACGTCTG77550.038.52755415
CACGTCT77550.038.41150714
CGTCTGA79650.037.5400116
CCGATGT77950.037.4097933
AGCACAC81250.036.80076210
CGTATGC80450.036.7762344
CAGTCAC79750.036.64990227
GTCACCG79500.036.6236429
CGATGTA80750.036.28151734
GTATGCC81800.036.19679345
CCAGTCA82200.035.77652426
TCCAGTC82650.035.71819325
GAGCACA84100.035.6339049
GAACTCC83100.035.6330821
TATCTCG82900.035.58350439
TCGTATG83400.035.5293543
Produced by FastQC (version 0.11.2)