FastQCFastQC Report
Thu 22 Sep 2016
HJKYNBCXX_l02n01_vs19_160909.35100000068a4a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKYNBCXX_l02n01_vs19_160909.35100000068a4a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7717565
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG11350.027.5534611
CGTGGCC11500.027.39165933
CACGTGG12100.026.03339631
AGTCACG12250.024.97959328
ACGTGGC13100.024.04611232
GATCGGA12950.023.6385331
ACACGTC13650.023.24040213
CACACGT13650.022.91075112
TCACGTG14000.022.82172630
GTCACGT13950.022.25835229
ACGTCTG14100.022.1795615
CACGTCT14850.021.21088414
CGTCTGA15250.020.65453316
GTGGCCT15700.020.49482534
GGCCTTA16200.019.44494836
AGCACAC17150.018.62865310
CAGTCAC17200.018.31454727
GAACTCC18200.017.55574221
TCCAGTC18900.017.26279825
TATCTCG18400.017.24162341
Produced by FastQC (version 0.11.2)