FastQCFastQC Report
Thu 22 Sep 2016
HJKYNBCXX_l02n01_vs18_160909.35100000068a20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKYNBCXX_l02n01_vs18_160909.35100000068a20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15109666
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGG23000.026.02192329
CACGGCT23500.025.4681831
GCACACG24150.024.9675511
TCACGGC24400.024.4366530
AGTCACG25100.023.8448728
CGGCTAC25200.023.8395333
GATCGGA25050.023.8116261
ACACGTC26150.023.14402213
ACGTCTG28300.021.70373315
ACGGCTA28000.021.69672632
CACACGT29150.021.14804812
CACGTCT28800.020.93633314
CGTCTGA30250.019.93277216
CATCTCG31150.018.92550739
AGCACAC33050.018.3121410
CAGTCAC33500.017.79898627
GAACTCC35550.017.21565221
GTATGCC35150.017.02675245
GAGCACA35700.017.0158589
CGTATGC36250.016.82047544