FastQCFastQC Report
Thu 22 Sep 2016
HJKYNBCXX_l02n01_vs17_160909.35100000068b12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKYNBCXX_l02n01_vs17_160909.35100000068b12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15229454
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC255250.16760285693761576TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG36800.033.4424711
ACACGTC39850.030.93934213
ACGTCTG40400.030.5738315
CACACGT41800.029.65747512
GATCGGA43200.029.0213181
CACGTCT43750.028.12989414
AGCACAC45050.027.61780710
CGTATGC46200.026.78548644
CGTCTGA46800.026.53701416
GTATGCC46650.026.52710745
CACTAGC46500.026.22584231
CAGTCAC47900.025.83561727
GAGCACA48500.025.5140749
TATCTCG49200.024.97077439
CCAGTCA51350.024.23127726
GAACTCC50750.024.11889321
TCCAGTC53100.023.43300425
TCACTAG53350.023.32258230
TCGGAAG54850.022.970493
CTCGTAT54200.022.91493442
Produced by FastQC (version 0.11.2)