FastQCFastQC Report
Thu 22 Sep 2016
HJKYNBCXX_l02n01_vs16_160909.35100000068a06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKYNBCXX_l02n01_vs16_160909.35100000068a06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7623593
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC1556912.0422260212474614TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG164300.043.68174711
ACACGTC164800.043.5082613
GATCGGA167100.043.1174131
ACGTCTG166200.043.0876215
CACACGT166800.043.02704612
CACGTCT166700.042.97187814
AGTCACG165850.042.77523428
CGATCAG166600.042.74641833
GTCACGA166750.042.55841429
AGCACAC168950.042.5327610
CACGATC167450.042.46140331
CGTCTGA169000.042.3471216
CGTATGC168400.042.22103544
GATCTCG168000.042.17672739
GAGCACA170700.042.1230859
GTATGCC169300.042.00932745
CAGTCAC169800.041.8210227
AGAGCAC172150.041.807498
GAACTCC170650.041.78549221
TCCAGTC170300.041.69987525