FastQCFastQC Report
Thu 22 Sep 2016
HJKYNBCXX_l02n01_vs15_160909.351000000689eb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKYNBCXX_l02n01_vs15_160909.351000000689eb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14156011
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC2544731.7976321154313881TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG274700.043.6706711
GATCGGA278800.043.1994821
ACACGTC277000.043.1781113
CACACGT278550.043.05912
ACGTCTG279250.042.7899215
CACGTCT281200.042.533214
AGCACAC284900.042.22562810
GTCACAC282200.042.09799229
CGTCTGA284200.042.06838616
GAGCACA287200.041.910979
TCCAGTC285800.041.5764725
AGAGCAC289800.041.558258
CGTATGC286050.041.55498544
CAGTCAC286500.041.4431827
CCAGTCA286950.041.41739326
GTATGCC286750.041.41401745
GAACTCC290700.040.99118821
TCGGAAG294400.040.9547543
CTCGTAT289650.040.8441642
AGTCACA291350.040.81478528
Produced by FastQC (version 0.11.2)