FastQCFastQC Report
Thu 22 Sep 2016
HJKYNBCXX_l02n01_vs14_160909.351000000689c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKYNBCXX_l02n01_vs14_160909.351000000689c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245661
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC21205386.31935879117971TruSeq Adapter, Index 3 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATTCGTATGCCG9800.3989237200858093TruSeq Adapter, Index 3 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATTTCGTATGCC5140.20923142053480204TruSeq Adapter, Index 3 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGTATGCCGT4050.16486133330076813TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCCAGTCACTTAGGCATCTCGTATGC3160.1286325464766487Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCAGT302.1585784E-644.99979825
CTCCCAG302.1585784E-644.99979824
AATTCCA302.1585784E-644.99979822
CATTCGT1150.044.99979839
ACTCCCA302.1585784E-644.99979823
CATTTCG551.8189894E-1244.99979839
CTGAACC302.1585784E-644.99979819
ATTCGTA1150.044.99979840
GAATTCC302.1585784E-644.99979821
AGGCATT1700.044.99979836
TGAATTC302.1585784E-644.99979820
ATTCCAG302.1585784E-644.99979823
GCATTCG1150.044.99979838
ATTTCGT551.8189894E-1244.99979840
ACACGTT453.8198777E-1044.99979413
AAACTCC207.0226047E-444.99979422
TCTAGTC207.0226047E-444.99979425
CAGTTAC253.8819286E-544.99979427
GGCATCG406.7775545E-944.99979437
GAAACTC207.0226047E-444.99979421
Produced by FastQC (version 0.11.2)