FastQCFastQC Report
Thu 22 Sep 2016
HJKYNBCXX_l02n01_vs13_160909.351000000689a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKYNBCXX_l02n01_vs13_160909.351000000689a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17528
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC605534.54472843450479TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC6400.045.013
CACACGT6350.045.012
CACGTCT6400.045.014
TCGGAAG6400.045.03
CGGAAGA6400.045.04
ACGTCTG6450.044.99999615
GATCGGA6450.044.9999961
GAAGAGC6300.044.9999966
GAGCACA6250.044.9999969
AGAGCAC6250.044.9999968
ATCGGAA6450.044.9999962
AGCACAC6300.044.99999610
GCACACG6300.044.99999611
CGTCTGA6450.044.99999616
AACTCCA6450.044.6511622
CTGAACT6450.044.6511619
GAACTCC6450.044.6511621
GATCTCG6450.044.6511639
TGAACTC6450.044.6511620
TCACATC6400.044.64843830
Produced by FastQC (version 0.11.2)