FastQCFastQC Report
Thu 22 Sep 2016
HJKYNBCXX_l01n01_vs8_160909.35100000068958.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKYNBCXX_l01n01_vs8_160909.35100000068958.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7388748
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG2515583.404609278865648TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG270900.044.0662711
GATCGGA274800.044.041971
CACACGT275000.043.49109312
ACACGTC274950.043.48263513
ACGTCTG274400.043.37301615
AGCACAC276000.043.3498210
CACGTCT275600.043.28212414
GAGCACA278550.043.0660489
CGTCTGA276500.043.0276216
TCGGAAG281850.042.9620933
AGAGCAC280950.042.771398
AGTTCCG275450.042.50369634
TCCAGTC277750.042.47578425
GAACTCC278600.042.45004321
TCGTATG276350.042.41010745
GTTCCGT276200.042.39642735
ATCGGAA286350.042.3340872
CCGTATC275750.042.2570338
CGGAAGA286650.042.1798974
CAGTCAC279800.042.139627
Produced by FastQC (version 0.11.2)