FastQCFastQC Report
Thu 22 Sep 2016
HJKYNBCXX_l01n01_vs7_160909.3510000006894b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKYNBCXX_l01n01_vs7_160909.3510000006894b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8969811
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG974321.0862213261795595TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG112750.041.68420411
GATCGGA116300.041.293621
ACACGTC115750.040.91482513
CACACGT116000.040.5939112
CACGTCT117250.040.2570814
ACGTCTG117550.040.15434315
AGCACAC118000.040.0584110
CGTCTGA119250.039.60099816
GAACTCC120750.039.00205221
GAGCACA122450.038.7312479
CACAGTC120500.038.69341731
TCCAGTC121450.038.61158425
AGAGCAC123450.038.472828
TCGGAAG125100.038.3610463
CAGTCAC122250.038.24911527
GTCACAG122050.038.2200329
CCAGTCA123250.038.03048726
TCGTATG122100.037.885845
ATCGGAA128150.037.5007172
AGTCACA124850.037.450728
Produced by FastQC (version 0.11.2)