FastQCFastQC Report
Thu 22 Sep 2016
HJKYNBCXX_l01n01_vs6_160909.3510000006893e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKYNBCXX_l01n01_vs6_160909.3510000006893e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8606746
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC892681.037186411682185TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG103500.042.23622511
ACACGTC105300.041.38604713
GATCGGA106450.041.3520471
CACACGT106600.041.05018212
ACGTCTG106800.040.78371415
CACGTCT107750.040.42413714
AGCACAC109350.040.0795510
CGTCTGA109400.039.89671316
CGTATGC108950.039.5897644
GTATGCC109150.039.5788345
GAACTCC110350.039.47650521
GAGCACA111050.039.4669
TCGGAAG112300.039.2475363
AGAGCAC112550.039.000448
TCCAGTC111500.038.96980325
CAGTCAC111250.038.87443527
CCAGTCA111400.038.84364326
AGTCACC111600.038.6304228
CTCGTAT112700.038.2500442
TCGTATG113100.038.23411643
Produced by FastQC (version 0.11.2)