FastQCFastQC Report
Thu 22 Sep 2016
HJKYNBCXX_l01n01_vs4_160909.35100000068921.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKYNBCXX_l01n01_vs4_160909.35100000068921.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11477084
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC224551019.565161324949788TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2450500.044.8291631
TCGGAAG2461350.044.5415233
ATCGGAA2471800.044.431492
GCACACG2435850.044.40019611
GAGCACA2448900.044.3739749
AGCACAC2442800.044.3659810
CGGAAGA2468950.044.3615844
AGAGCAC2452950.044.3556028
ACACGTC2440750.044.32119813
CACACGT2440250.044.32106412
CACGTCT2442350.044.2101814
ACGTCTG2447700.044.16686215
GAAGAGC2475750.044.122516
CGTCTGA2451650.044.09937316
GGAAGAG2485800.044.0916525
GTATGCC2441550.043.942845
GTCTGAA2467000.043.8933817
GAACTCC2443200.043.88134821
TCTGAAC2458050.043.876218
AAGAGCA2489200.043.8714457
Produced by FastQC (version 0.11.2)