FastQCFastQC Report
Thu 22 Sep 2016
HJKYNBCXX_l01n01_vs3_160909.35100000068914.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKYNBCXX_l01n01_vs3_160909.35100000068914.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9602347
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC1148661.1962283804157463TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG126850.043.0989811
ACACGTC129750.042.2570713
GATCGGA130850.042.1866041
CACACGT130950.041.8182912
ACGTCTG130350.041.7691415
CACGTCT132400.041.2753514
AGCACAC133750.041.06059310
GTCACAC130750.041.02852229
CGTCTGA133000.040.97094316
CACACAG131150.040.81887431
GATCTCG132000.040.55074739
CAGTCAC133250.040.32609627
GAGCACA136400.040.2463659
CACAGTG133500.040.04726833
AGAGCAC137750.039.8527688
GAACTCC135900.039.78645721
CGTATGC135700.039.69421444
TCGGAAG139600.039.5824973
TCCAGTC136300.039.57126625
ACTCCAG137450.039.2727223
Produced by FastQC (version 0.11.2)