FastQCFastQC Report
Thu 22 Sep 2016
HJKYNBCXX_l01n01_vs2_160909.35100000068907.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKYNBCXX_l01n01_vs2_160909.35100000068907.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10341202
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC452420.4374926628451896TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG52200.040.29887811
GATCGGA55800.038.1927571
ACACGTC56150.037.38382713
ACGTCTG56400.037.21811715
CACACGT57050.036.83350812
CACGTCT57450.036.53788814
AGCACAC58000.036.3077810
CGTCTGA58200.036.0672216
ACTGACC57850.035.86351832
CGTATGC61250.033.7223544
GTATGCC61700.033.69471445
GAGCACA62750.033.631099
GAACTCC62800.033.42906621
CAGTCAC62500.033.1581527
TCCAGTC63150.033.10206625
TCGGAAG65300.032.766473
AGAGCAC64500.032.684538
CACTGAC64000.032.6633831
CCAGTCA63900.032.57300626
ACTCCAG65750.031.86122523
Produced by FastQC (version 0.11.2)