FastQCFastQC Report
Thu 22 Sep 2016
HJKYNBCXX_l01n01_vs26_160909.351000000689f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKYNBCXX_l01n01_vs26_160909.351000000689f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9525061
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG11100.018.2419211
CTTAGGC11900.015.69403333
ACACGTC12950.015.46219813
GATCGGA13400.015.4504521
CACACGT13350.015.167438512
ACGTCTG14800.013.68143915
CACGTCT15000.013.49902114
CGTCTGA15700.013.32705916
ACTTAGG14600.012.63819232
CATCTCG16250.012.04610239
AGCACAC17400.012.0249910
CGTATGC17150.011.54532144
AGAGCAC19650.010.8774738
GTATGCC18550.010.79498845
GAGCACA20100.010.745499
TAGGCAT20200.010.58224835
CAGTCAC20100.010.41127827
CTCGTAT20200.010.35864842
TTAGGCA19450.010.18043934
GCATCTC20750.010.08429438
Produced by FastQC (version 0.11.2)