FastQCFastQC Report
Thu 22 Sep 2016
HJKYNBCXX_l01n01_vs25_160909.351000000689de.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKYNBCXX_l01n01_vs25_160909.351000000689de.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8169053
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC96170.11772478401107203TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG15550.031.25123411
GATCGGA17850.027.4810851
ACACGTC18050.026.9228113
ACGTCTG19050.026.10003915
CACACGT19050.025.86383812
CGATCTC18450.025.24366238
CACGTCT20200.024.50276814
CACGATC19550.024.2842536
GATCTCG20000.024.18712239
CGTCTGA20700.023.47631316
AGCACAC21500.022.91656310
CGTATGC21850.022.44898444
CATCACG21300.022.29029533
GAACTCC22600.021.80395721
CAGTCAC22750.021.26561727
GAGCACA23700.020.7892869
GTATGCC23400.020.57685545
TCCAGTC23900.020.5244525
CTCGTAT24600.020.3960242
AGAGCAC24600.020.2127278
Produced by FastQC (version 0.11.2)