FastQCFastQC Report
Thu 22 Sep 2016
HJKYNBCXX_l01n01_vs12_160909.351000000689b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKYNBCXX_l01n01_vs12_160909.351000000689b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10267415
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG771710.7516107997972226TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG89000.041.17962611
GATCGGA92400.040.38091
AGTCACG90550.039.7326328
ACACGTC92500.039.57283813
ACGTCTG93200.039.25147615
GTCACGT91950.038.98427229
CACGTCT93650.038.9667714
CGATCTC91600.038.93139640
CACACGT94050.038.8967312
GATCTCG92400.038.76477441
AGCACAC95100.038.75116310
CGTCTGA94550.038.5720616
TCACGTG95150.037.8152730
CACGTGA95250.037.77556631
GAGCACA98500.037.5049259
TCGGAAG99800.037.3781663
CAGTCAC96700.037.29984727
TCCAGTC98150.036.9125
AGAGCAC100950.036.5957688
CCAGTCA99500.036.45479226
Produced by FastQC (version 0.11.2)