FastQCFastQC Report
Thu 22 Sep 2016
HJKYNBCXX_l01n01_vs11_160909.3510000006899b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKYNBCXX_l01n01_vs11_160909.3510000006899b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9891976
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG1466211.4822215500725033TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG164350.042.69678511
GATCGGA170450.042.250521
ACGTCCG163750.041.6663332
CACACGT168750.041.5301812
CGTCCGC164350.041.4426133
ACACGTC168800.041.35793713
AGCACAC170600.041.27763710
ACGTCTG170200.040.99130215
CACGTCT170700.040.85805514
CGTCTGA170750.040.83333216
CACGTCC167500.040.69319531
GTCCGCA167650.040.66712634
CCGCACA167500.040.63329736
AGTCACG168500.040.62243728
GAGCACA173900.040.5848969
GTCACGT170200.040.2720129
AGAGCAC176100.040.1942838
TCGGAAG179550.040.1236843
TCACGTC170850.040.01383230
TCCGCAC171350.039.7889935
Produced by FastQC (version 0.11.2)