FastQCFastQC Report
Thu 22 Sep 2016
HJKYNBCXX_l01n01_vs10_160909.35100000068971.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKYNBCXX_l01n01_vs10_160909.35100000068971.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9245561
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG1873442.0263129516964953TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG213600.043.39615211
CGTCCCG208200.043.3160935
GATCGGA217600.043.1482161
GTCCCGA209650.043.01417536
ACACGTC217000.042.7991613
TCACCCG212650.042.7397430
CCCGTCC212100.042.71034233
ACGTCTG217300.042.65723815
AGCACAC217850.042.64249410
CACACGT217750.042.6414112
ACCCGTC213550.042.47531532
CACGTCT218500.042.45385714
CCCGATC211700.042.40565538
CACCCGT214950.042.31382431
CCGTCCC214450.042.24230634
GAGCACA221150.042.1689579
CGTCTGA220850.042.0023516
GTCACCC217400.041.83696429
TCCCGAT215900.041.82108337
AGAGCAC223400.041.765528