FastQCFastQC Report
Fri 15 Jun 2018
HJKMGBCX2_l02n02.332000000e7bc9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKMGBCX2_l02n02.332000000e7bc9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177496306
Sequences flagged as poor quality0
Sequence length99
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCA7993530.45034909064530054No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG6334630.3568879906717608No Hit
GTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGGTGCAATTG3922220.22097473960951053No Hit
GGTGCATATGGTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCT2427120.13674200070394704No Hit
GCTAAATAAACAAGAAAGCAAAATCAACAACCGCAAAAGTCTAAGACATG2137330.12041546374491872No Hit
GGTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGGTGCAATT2053180.1156745200094474No Hit
GCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGGTGCAATTGCA2043330.11511957888295433No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCAGC1791930.10095590383723255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT7859100.075.955841
AGCAGTG8123450.073.30182
CAGTGGT9066700.065.597554
GCAGTGG9380550.063.492313
AGTGGTA9363600.063.4911885
GTGGTAT9896750.060.111526
GGTATCA10069100.059.0354548
TGGTATC10091900.058.942577
GTATCAA12236400.048.8148889
CATGGGG6271150.032.90708524-25
AGAGTAC10012300.032.79034418-19
GCAGAGT10102400.032.51935616-17
TACATGG10023550.032.35941322-23
CAACGCA10432850.031.76246512-13
ACGCAGA10432200.031.73062314-15
ATCAACG10554450.031.51171310-11
AGTACAT10759250.030.36568620-21
CATGGGA3352800.029.26045624-25
CAGAGTA10122550.029.13592318-19
CGCAGAG10181950.029.10941316-17
Produced by FastQC (version 0.11.2)