FastQCFastQC Report
Fri 15 Jun 2018
HJKMGBCX2_l02n01.331000000e7bcc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKMGBCX2_l02n01.331000000e7bcc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177496306
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCGAC307600.018.4776978
CAATGGA566150.018.4504748
GGAAAGC453300.018.2436071
CTGCGGA422150.018.0560631
GATGAAA519350.018.028191
ATAACGC519600.017.9102841
GACTGCG518350.017.8338381
TTGAACG473400.017.777621
GCGCGAT411200.017.7751661
GGGAATG456050.017.6887861
CATGGCG554900.017.636551
TGAGCCG377450.017.6297021
GGGCATC584350.017.6182791
ACTGAAC441200.017.5980531
ACTGATG450700.017.5442811
TGACAAC488700.017.5372071
TGACGGC376400.017.516921
TGGCCAG523850.017.4158571
GACCTGG513200.017.3942831
CTCGAAA403000.017.3909231
Produced by FastQC (version 0.11.2)