FastQCFastQC Report
Fri 15 Jun 2018
HJKMGBCX2_l01n02.332000000e7bd6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKMGBCX2_l01n02.332000000e7bd6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203968309
Sequences flagged as poor quality0
Sequence length99
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCA10298460.5049049065754622No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG8608130.42203271881809834No Hit
GTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGGTGCAATTG6152520.30164097698138No Hit
GGTGCATATGGTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCT3302470.1619109368602943No Hit
GGTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGGTGCAATT2881900.14129155720950748No Hit
GCTAAATAAACAAGAAAGCAAAATCAACAACCGCAAAAGTCTAAGACATG2788210.13669819658111693No Hit
GGTGCAATTGCAAAGGCACAGGAGCACAGGAGCAGCTAAATAAACAAGAA2639500.1294073580812988No Hit
GCAAAATCAACAACCGCAAAAGTCTAAGACATGTATCACGAATTGAAAAT2166570.10622091297526029No Hit
GCAAAAGCCAAGGCCGGAGCAGGTGCATATGGTGCCAACCCCCCTGGCCC2132050.10452849319842134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT6616350.078.338414
AGTGGTA6645700.078.032725
GTGGTAT6884850.075.3455356
GGTATCA7069900.073.398988
AGCAGTG7105750.073.139782
TGGTATC7107350.073.099147
AAGCAGT7349400.071.135991
GCAGTGG7339100.070.765923
GTATCAA9579700.054.4901439
CATGGGG4954250.039.7984224-25
AGAGTAC7040750.038.82047718-19
GCAGAGT7080000.038.63302216-17
TACATGG7142750.037.77662722-23
ACGCAGA7433550.036.99727614-15
CAACGCA7453250.036.9942212-13
ATCAACG7613600.036.35734610-11
CGCAGAG7181950.036.180916-17
CAGAGTA7169150.036.1210718-19
ACATGGG7075000.036.04667724-25
ATGGGGG3383350.035.9242326-27
Produced by FastQC (version 0.11.2)