FastQCFastQC Report
Fri 15 Jun 2018
HJKMGBCX2_l01n01.331000000e7bd9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKMGBCX2_l01n01.331000000e7bd9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203968309
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGGA534900.018.5925241
CAATGGA543950.018.0623938
GGGACCT613850.018.0160561
GATGAAA560900.017.7981991
TGCTACC714000.017.7652931
GACTAAC504850.017.7196941
TGACGGC449350.017.66791
TGGACGC576450.017.6556871
ACTGAAC472700.017.4694561
CGTGAGC650750.017.4147721
GGAAAGC551850.017.3599951
GCGCGAT431200.017.3064381
TTGAACG453600.017.2797261
AGGCGAC227500.017.2314288
CTGAAAC537200.017.1500031
GCATGCG535750.017.1492961
CTCGAAA475200.017.1428971
GGGAATG458600.017.1073861
GATCTAG671950.017.1032941
ACGCCGA566950.017.0516381
Produced by FastQC (version 0.11.2)