FastQCFastQC Report
Fri 18 Mar 2022
HJKC5AFX3_n01_T6_Rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKC5AFX3_n01_T6_Rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCAAGCAAAGCACACATCCT27.6923076923076925No Hit
CCGGGAATCAACAAACTAGGACACGTTCCTAAAAAATAACCAGAAAAAAG13.8461538461538463No Hit
GGTTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13.8461538461538463No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGGCCGGGCTGGGAAAA13.8461538461538463No Hit
CACTGGAAAAAAAAAATACAATAAAAAAATAAAGAAAACTAAATACAAAA13.8461538461538463No Hit
ATGCGAATTCACATCACAAAACTATCAACGGTAAACCGAATCAAGATACT13.8461538461538463No Hit
TGGAAAAAAGGGGTTAAAATACAGGGTAACACATTCCAATGACATAAAAG13.8461538461538463No Hit
GGCCATATATGAAGAAAAACAGGCCCACTCTAGACACTTGTCATAAAACG13.8461538461538463No Hit
GGAGTGTGGTTATGGCGCTGAACGGTCTCAGCGTAAGTCCGGACTTTCCA13.8461538461538463No Hit
GGGGCACACACGGATGAGTGAATGCGGCTGGCAACAAACTGTAACTATTG13.8461538461538463No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTGAGGACCAGGACTACA13.8461538461538463No Hit
GGCGACAAAACTCAGAGAAGACCGCAAATAAACAAGAAACAACCAAAACC13.8461538461538463No Hit
GGTAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13.8461538461538463No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCTGATATGTTAGCTCAG13.8461538461538463No Hit
CGTGCAAAAAAAATTTAATATAAAAAAGAACTAAAGGTAAAGAATAAAAA13.8461538461538463No Hit
GGGGGACTTAAAAACCGATCTAAAAAAGAAGCTGAATCAAACGTGTAAAA13.8461538461538463No Hit
ATAAAAAAAAAAAAAAAAAAAAACAAAACATAAAGAAATAAAAAAAAAAA13.8461538461538463No Hit
ACGAAAAATGTAAACATAATACTACTAAAAAACCTGCAACCCCCATAACA13.8461538461538463No Hit
GGGACTAATTACCGAGTAATTTTCCCATACTATGCTACATTAGAGAAAAT13.8461538461538463No Hit
CCCGGCATAAAAAAAAACCTAAGATTTGAGACTTAACGAGTTCTGTCAAT13.8461538461538463No Hit
TCGGCGAGCGGGGTATGCCGATGTGGGGCTGATGGTGGGGTCCGTGGTTG13.8461538461538463No Hit
AGGTGAGGGCCGCACAAGCAGCCAAACTAAGCTCAACGCTAAAATGAATT13.8461538461538463No Hit
GGGGTGTGGGGGGGGTGGGGGGGGGGGTGGGGGGGGGTGGGGGGTGGGGG13.8461538461538463No Hit
GGGGGGCATGCGGTTATTTGTGAGTGTGTCTGGTCGCTATCGGGGTGAGT13.8461538461538463No Hit
TTACAGAGGCTAAAATTGAATAAAAAAAATAAAGAGATAAAAACACGAAA13.8461538461538463No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)