FastQCFastQC Report
Fri 18 Mar 2022
HJKC5AFX3_n01_T6_Rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKC5AFX3_n01_T6_Rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAGAGATTAAGTAAAAGGAATCCCTGACAAACAAAAACAAAATCACG11.639344262295082No Hit
GGGGGTCGGGGGGGTGGGCGTCGGGGTGGGGTTGGGAGGGGGTGGGGCGG11.639344262295082No Hit
CGTGGAAACCTCAGACCGAAACCCCCATACAAAACCAAAACCTCAACAAA11.639344262295082No Hit
GGGCACAGGGCCCGCGGGTGGGTATACAGTCCGATGCATAATATATGTTT11.639344262295082No Hit
TTAGTAGGAGAGAACGCATGGTGATCGACAATGAAAGTCGATGACGGGCG11.639344262295082No Hit
GGAGCAAACAAGAGGGGGTGACCACGCTTGAACTCAGAATGCGAAAACGG11.639344262295082No Hit
GGGCTAAAGGCCTGAGTCGAAGCCCCGGATAAAAAGAAGAACAGAACCAC11.639344262295082No Hit
GGGTCTAAACACAAGCGGCACTAGGGGATCAAACAAAATAGCAAAAAAAG11.639344262295082No Hit
GTGCCGAAAAAACGCAAGACAATAAACCCAAAAAGACAATAACTCAAAAA11.639344262295082No Hit
GGGGCGGAAAGGGGGGGCTATCCCGGGCTATTCGAAAAATCGATCAAACG11.639344262295082No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCCACACCCAAGGAAAAG11.639344262295082No Hit
GGGGATAAAAAGGCGGGCAAATGAACAAGCAACACCATAACGATAAAAAG11.639344262295082No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTGAGGAGCTCACGCTG11.639344262295082No Hit
GGGTAGTAATAGGCGGGGACGAAACCCATCTTAGAGAAAAAGACTTTAAT11.639344262295082No Hit
GCAGAACCCCTCTACGAGAGCGAAAGAACCGCTCGAATTAGTACTTCACA11.639344262295082No Hit
GTGGTAAATTGGTTAAATCAAAATGTGCAAAAAACAAAACGCCAAAACGA11.639344262295082No Hit
GGTGACTTAAGCGGGGGAAAAAAAAAGTAAAAATCACAAAGCAGACAACA11.639344262295082No Hit
GGGGCAAAAAAGGGGGGAGAAACGGCGCATAAATGACAAAACTGAAAAAC11.639344262295082No Hit
GGGGCCGGGGGGGGGGGGTGGGGGGGGGGGGGGGGTGGGGGGGGCGGGGG11.639344262295082No Hit
GGGCTTGAAATCCAAGAAACATACACCTAATTAGAGCTAATCTTCTACAG11.639344262295082No Hit
GGCGAAAAAAGGCGGGGGTCAGCGACGAAGAACAGTAAGACCGAAAAACC11.639344262295082No Hit
GGGGAGTTGGGGGCGGTCAGGGGGGGGAGAAAGCGACACGGGGGGAACGG11.639344262295082No Hit
CCGGGGTCGCCCAAGTCCCTGCCCCAGACCACTTAGCACAAATTAACCGA11.639344262295082No Hit
TGGGNTAGCCGTCTAAGAATATAAAAAAACAGCAATGAAAACAAATAACT11.639344262295082No Hit
GTCAGCTAACTAAGAAAATAACCAGACAGTATCATAATACAGTACGCACA11.639344262295082No Hit
CCGAGGATACTGCGACAAGCCCGAACCACATCAAGCGTCAAAAGCAACAT11.639344262295082No Hit
AAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGGTGTGAAAGTAAAGGA11.639344262295082No Hit
GGGGAACATAAGGCGGGGCGATCGTGGTACCACAAACCTACGCACTAAAA11.639344262295082No Hit
GGGGGTCGTAACCATGGAGACAAAATAGAAACCTAGCATCATTAAGGAAG11.639344262295082No Hit
GGGGAAGAAAAAGGGGGGAGAACCCCGAAACAGACGGAAAGTTAGAAGTC11.639344262295082No Hit
CAACAGAAACACCAACAGAAAAAAACCAAATAAAAAAAAAAAAAAAAAAA11.639344262295082No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAAACAAGACGGGTGGAG11.639344262295082No Hit
GGAACAAACAACACCACAGCAAAACAACCAAAACTAAGAATACTTAAACA11.639344262295082No Hit
CCCTAAAATAAGTACCCGACTAAATAACGGATCCAACTAACAAAAAAGCA11.639344262295082No Hit
GCGGAAGAAAGGAGTGCGTGCACAATAAATGAACAATCCAAAACATCAAT11.639344262295082No Hit
CCGCATAAGATCGCACGGAAGAGGGCTAATCTGAAGCATGCATTAAAAGC11.639344262295082No Hit
GGGCAACCCAAAAGGTTGACACAATATAGGATAAGAGTAATTCCAAAGAA11.639344262295082No Hit
GGCGGTTCGTAAAAAAGAAAGAAATAAGAACCGCGGATCTAACTAAAGAA11.639344262295082No Hit
GGGGCTAGGGTCTCACTAAACTTAAATATATAGTTATACAAATTAAGTTT11.639344262295082No Hit
TTACACGATCTCTTGTGGAAAGGACGAAACACCGCATGGATGGTTATGGG11.639344262295082No Hit
TGGAGATATTTGCCGAGTGATCTGGGGCACTTTGTAAAAAGGAAAAAATG11.639344262295082No Hit
GGGCTAACTAAGGCGGGTAAAAACAGAAAAAGAACCAGAAAGAACCTAAA11.639344262295082No Hit
GGGTGAAAAATTGGGGAAGTAACCAACGACTACCAAAAAAAGCCAAAAAC11.639344262295082No Hit
TGGGGAAGAGAAAACGCGTACAAAAAACCCAGAAGCACCAACACAAGAAA11.639344262295082No Hit
CTCTATGTTGCACACCAAGAAGAAAGGCGTGACATAGTCAGGGACCAACC11.639344262295082No Hit
GGGTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.639344262295082No Hit
AAAACACAAAAAAAAATACCAAAAACAACAAAAAAAAAATAACAAAAAAA11.639344262295082No Hit
GCGGCTAACTCGGTGCGCTACTAGCCGCATAAGAAACAATGGTAACCAGG11.639344262295082No Hit
TGGCCAAGAGAGATCCGACAGATGACCACTAGTACCCGAAGGAGCGTTTC11.639344262295082No Hit
CCCGCCAAAAAGGGGCCGTAAATGCTGAAAAAAAAAACATGGTTAAAAAT11.639344262295082No Hit
GCGGGCTAAAGAGGAGGCTAACGGAAGGCCAAACCGATCAGCGTCAACTG11.639344262295082No Hit
GTCAGTCAAATCATACATTTAATAATACCCAACTCACTTTAAACTTAATA11.639344262295082No Hit
GGGTAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.639344262295082No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGTTCTGGTCCTTGTTC11.639344262295082No Hit
GGGCTTCCGAGAGGGGGGTGCAAAAAGAAGCGCACTAGCTAATTAGGGTA11.639344262295082No Hit
AACATACCAAAAAAATAATACTGAAACTAAAAAGTACTTTTTCAGAAAAT11.639344262295082No Hit
GCACCAAGAAAAAAAGGGCACAAATCATAACATAGAAATTAAAGAAGAAA11.639344262295082No Hit
GGGGCACAAAAAGAGGGGAATAAATTGCACCAACGTATATTTACCAAAAG11.639344262295082No Hit
NACATACAAAAAACACCACCGAAAAAAACTCAATAAAAAATAAACATGCA11.639344262295082No Hit
GCGCGACATAAAGTGGTGGATAACATCCTCCCATCTCACCTTGAGCAAAC11.639344262295082No Hit
GGGGCATTTAAGCAGGGGCAGCAGCTTGAAAGAGATATTAGACACTAAAG11.639344262295082No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)