FastQCFastQC Report
Fri 18 Mar 2022
HJKC5AFX3_n01_T18_Rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKC5AFX3_n01_T18_Rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCCAAATTTGCTATCACC24.651162790697675No Hit
AAACAAGCAAAAACAAAATAACAAAAAGAAGAAAAAAGATAATAGGATAT12.3255813953488373No Hit
AATACGAGGATCGAGCCACCAAAGTACCTGACAGCTAAGGACTATCTCCA12.3255813953488373No Hit
CACCGATAGAAAAAAGCATAAAATAAATCTAATGCAGAAAACACTCTAGA12.3255813953488373No Hit
GGGGTCAAAATATAAAACCAAAGACCCAATAAGTAAAAATGAAATAAGAA12.3255813953488373No Hit
GTAATAAAACATAAAACACCACTCGAGAATAAAGACAAAACAGTACTACA12.3255813953488373No Hit
GCGGAAGAAAAATACACAAACAAAAAGACATTTACACGGTATAAACTAAC12.3255813953488373No Hit
CGCCCACGCTTTTAAAAAGTTTAGATACTACGATCGCTTTACCTGTGGGA12.3255813953488373No Hit
GGCTAAAAAACCCACAACAAAAATTACCAACAAATATAACATTAAAAACC12.3255813953488373No Hit
GGCGCTGGGGTGTGGGGGGTTGGGGGGTTGTTTGTTGTTGTGTTGTTGGT12.3255813953488373No Hit
TGGCTATAAAACCGCGCAAAAACCCCCCACAATACTAAAAAGCACAAAAA12.3255813953488373No Hit
GGTTTTGGGGTGTTTTGTGGGTTGTGGTGTGGTTTTTTGGTGGTGGTTTT12.3255813953488373No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAATAGCTCAGGACTTT12.3255813953488373No Hit
ACCAAAAACCGGTGGGAAAAAAGCCACAGCTAATACGATGGAAGATGCGA12.3255813953488373No Hit
GTTGTAAACCCATAGGGAGACCATAAACCGTAAAATATAAATTGTCTCAA12.3255813953488373No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGCATGTGCCTCCACGC12.3255813953488373No Hit
GGGGTCTAAAATTTCAATTTAAAACCGCACACAAACTACACTCATAAACA12.3255813953488373No Hit
GGGGTGGTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12.3255813953488373No Hit
TTCAATTCCAAGTATGAAAATTTACATGAAATAAACAACAGATTATGATA12.3255813953488373No Hit
TTTTGCACATAGTATGGTCAATCGCAACTGACTCTCTTCGACCAAAGCAA12.3255813953488373No Hit
ATAAGAAAGCTTAAAAACGTAGAAAAATACAACCTAAACGTAAAAAACAA12.3255813953488373No Hit
AAAATAGCAACAAAAAAACCAAAAAAAGCTTTAATAACCAAAATAGAAAA12.3255813953488373No Hit
GGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12.3255813953488373No Hit
AAACGAAAATAAAACACCGACTAAAAAAAAACACCGAACTAACCAACTAA12.3255813953488373No Hit
GGTTCTGGGGGGTTTTTGGGTGTGTGGTGTTGTGTTTTGGGTGTGTGGTT12.3255813953488373No Hit
GGTGATATATAATATTATCAATATAGATAAAGACACACAGGTGAATATGA12.3255813953488373No Hit
GGGGCTTCCTAGGGGCCTTCCAATCATAGGCAAACACAAAAGTGCATAAC12.3255813953488373No Hit
AAACACAAAATAATACTAACATAAAAACAACAACACACATAAAGTTAATT12.3255813953488373No Hit
GCGCAAGGAAATCATAAACCCTTATCACCCGAGCCATAGTAAGCAGAATT12.3255813953488373No Hit
GGATCTGGGGTGTTTTGGGGTGTGTGGTTTTGTTTGGTTGTGTTTTTTTT12.3255813953488373No Hit
TCTGGGAATAAATAATTACATAAAATATTACACAATTCTCAGTGCAAAAA12.3255813953488373No Hit
AGCGGAAAAAACCAATTACAAAACATCAAAAATAATACCGACAAGAAAAA12.3255813953488373No Hit
GGGTAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGG12.3255813953488373No Hit
CTACGACAAATTAAAACAGTATAAAAAGACACAATAACAAAACTTATCTA12.3255813953488373No Hit
AGGGGGCCGCTGAAAAATCAGACAAAACTTACAAAGTAAAGACAAAGTAT12.3255813953488373No Hit
CCGGGACATTTAAATCCAGAAAGAAATCGAAATATATAATCTTTAACCCA12.3255813953488373No Hit
GGGGGTTGTTGAGGGCGTGTTGTTTGGGTGATGTGCGGTGGCACAGTTTG12.3255813953488373No Hit
AGGGTTTAAAACCCGGAATTAAATGCAAAAAAAAACTAAAAAAAAAAACA12.3255813953488373No Hit
AAAGGGAATCATAAAATAGCTAATTCTCGATCTCCACGGAAAGTGTGATA12.3255813953488373No Hit
CGGGTCCGGGGGCCGGACAAGGGTAGCCAACGAGTCCCAGTGAGAGCGGA12.3255813953488373No Hit
NTTAATAAAAAAAATAAAATAAAAAAATAAAAAAAATAAAAATAAACAAA12.3255813953488373No Hit
TGGGGAAGTATGAGAAAAGATTAAACAAACGACAACTGGAAAAATTGAAA12.3255813953488373No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)