FastQCFastQC Report
Fri 18 Mar 2022
HJKC5AFX3_n01_T18_Rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKC5AFX3_n01_T18_Rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23.225806451612903No Hit
TATGTGAACAATAAAAGGACAAAAAAATCAACAGCTAAAAAACATAAAAT11.6129032258064515No Hit
GGGGGTAGGCGGGGCGCGGGAGGGGGGCAAAGCTATAAGCGGCTAGGTGG11.6129032258064515No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGGCTCGGGCGACATGG11.6129032258064515No Hit
TTGTAAAAAAATGGATAAAAACAAAAAGAAAAAACGGAATACGGCCAAAA11.6129032258064515No Hit
GGGGGGTGGAGGGGGGTGGGTCAGGGGAGGTTCCGGTAGGAGGGGCGTGG11.6129032258064515No Hit
GGGGCCGAAAAAACCTGCGTAAAACTCGAGACAGAAAAAAAAGTAAAAAA11.6129032258064515No Hit
CCAGCTACTAACATAAATCATAAAAACCCCAACTGCCAAGAAACTAAAAA11.6129032258064515No Hit
AACTCATTAAAAACACCTTCGGAATAAACTAAAAGCCTTAAAAGCAATAT11.6129032258064515No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTCTATAACCTCCCCGA11.6129032258064515No Hit
GAAGTAGAGTCTTGTGGAAAGGACGAAACACCTATATGCCACAGGAAGTT11.6129032258064515No Hit
ACCACCTAAACCTAAAGTAAAAGATAACACAAAAAAATACCCACTAAAAA11.6129032258064515No Hit
GGTGTAATAAAATAAACAGAAACTCAACAAATCAAATCAAAATCAAAAAA11.6129032258064515No Hit
ACGAAACAACAGCGAAACGCAAACACACACTACATAATACCCCTTATCAA11.6129032258064515No Hit
AGGTAATAGCGCGTGGGAAACCTGGGTTCCATCAGGAAGGGGGGATTTCC11.6129032258064515No Hit
GTGCCTTTTAACGGGGCGTTATAAAGGTGTACAGTAGTCAGGTAATTTGC11.6129032258064515No Hit
GCAATCGTTTCCATACTAAAGTTCAAAACGTATAAACGTTTAGTCGTTGA11.6129032258064515No Hit
AAAGGTAAAATTAAAAAGCTGAAATAATTAAAAGGTTAAATACTAAAATA11.6129032258064515No Hit
TTTGAGAAGTTACATGAAAAACTCAAATAAAAATACCCAAAACAAAGATC11.6129032258064515No Hit
CAAAAAATTAAACAAAAACCGTAAAAACGAGTAGTAGATAATCTCTGAAA11.6129032258064515No Hit
TCCGTCACTCTCATACTGCGATAATAAACCAGCGACACAAAATCATTAAA11.6129032258064515No Hit
GGGGGGAAAATGTATGGACGATGGAGGGCTCACACTCTAATCGTCAAAGC11.6129032258064515No Hit
AACAGTTAAATAATAAAAAGAGCCAAAAACGATATGGCAGAAAGTAACAA11.6129032258064515No Hit
CCTAGAAAATTTAGCAATGCTAGGGTTAGGATTACACAAAAAGCAAGTAA11.6129032258064515No Hit
ACGCTACAACAAACCACAAGATTAAAACTGTAGTGGCATTACGTAATATA11.6129032258064515No Hit
AACTGGAGCCCAAAATAAGGGGAAAAGGCATGACAAATGAAAAAAGGGCA11.6129032258064515No Hit
GGGGGGGTATGTAGTTTTGGTCGTTTGTCCAGGTGCGGCGTCAGGGGGCG11.6129032258064515No Hit
CAAATTGGGCTTATACAAACGTTATAAATCCGCGTGAGCAAACCACGCGG11.6129032258064515No Hit
AAAAATTATAATATAACAATACCTCAAAACAACAAAAAAAAATAAACTTC11.6129032258064515No Hit
AAAACGGTCAAAAGCCAAGAATTTAAAGTCGAAAAACTCTAAGTGAATTA11.6129032258064515No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGTGGAACTTGAGAAAGT11.6129032258064515No Hit
TTTGGGAAAAAAATGAAGGATAAACAACAAAAATGCAGCAACACAAAAGC11.6129032258064515No Hit
GGGGGCAAAGGCCCGAAAAAAAACGGTTTATAGATTAAAGGGCTAAATAA11.6129032258064515No Hit
AACCATAAAAAAAACAAAAAACAAAAATTCAAAGAGAAAAATAAAAAAAT11.6129032258064515No Hit
AAAATATTTTAAAAAATATTTTTTAAAGATATATCGGTTTTATACTTTTA11.6129032258064515No Hit
CAGCAAGAATAACATCGAAAAAACACCAGACATAATTCAGGCGACGAAAA11.6129032258064515No Hit
TACAAGCTTAAAAACCAACATAAAAAAATCTTATAGCCCTAAACTCTTTA11.6129032258064515No Hit
CTAGTCAGAAAAAATAAAAAAAATATGACGAATATATAAAAAAAACAACA11.6129032258064515No Hit
GCCTCATTCCAAACAAAACATACGCTAACAACAATGTGCCACTAACATAA11.6129032258064515No Hit
TCTGAAAATACAGGGACTAAAAGTCCGTTTTACATAATAAGTAAAGCTCC11.6129032258064515No Hit
CCTAAAGAAAGTATGGTCAATGGAAATAATCACAATACCAACATCAAACA11.6129032258064515No Hit
GGGTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.6129032258064515No Hit
ACACACCCCCAAAACTAATAACAGATCCAACAAAGGACACCTATCAAAAA11.6129032258064515No Hit
ACGACACTACAATAACAACTTAGAGAACAAATGCCTTTGTCAGTTGGAAA11.6129032258064515No Hit
GTTAGACTACGTCACGAGCCTGAGGAAGCGACGTGACAGTTATTGTCCGC11.6129032258064515No Hit
GTAATAATTTTTAAAAAAGATGTGATAAAGTTTTGGCATGGGACATGTTT11.6129032258064515No Hit
AAGGAAGGCAAATAAAGGAGGAAAAATAGAGCAAAGCTAAAAAATTTTAA11.6129032258064515No Hit
GGGGAGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGTGGGG11.6129032258064515No Hit
GCCCACTATAACCACCTCCATAAATCTAAAATAAGCATAATCAGTGAACA11.6129032258064515No Hit
GGCGTGGGGGGGGTGGTGGGTGTGGGGTTGTGGGTTTTTTGTTGTTTGTG11.6129032258064515No Hit
ATATAAATTAAAATTAAAAAAAAATAAGAGGTAACTGGATAACTGAAATA11.6129032258064515No Hit
AGGCGATACAACGAAAAATACCAAAAACCAAGAATAAAACAACGAAACAC11.6129032258064515No Hit
AAAAAAACTAAAAAAAAAAAAAAATAAATATAATGACTATTAAGGAAAAT11.6129032258064515No Hit
TCTAAAACACAAAAATATAAAAAAAAAAATACCAAACACAAAAAAGGCAA11.6129032258064515No Hit
GGGGGAAAAAAAGTGAACACAAAAACCAAAAAAACAATATCAAAAAAAAC11.6129032258064515No Hit
AGTGGGGGGTGTAGATACTTGGTTGGTCGGGGTGAGATAAGGTGGTGATG11.6129032258064515No Hit
GCCGCTATTGTCATCAATATTGGAGAATGAACTGGCCATTACCAAGTTAA11.6129032258064515No Hit
GTGTAGACCGCGGGCCAAAGCACGGTGTTAACGTCCCAACGGAGAAACCG11.6129032258064515No Hit
GGGGTCTCCAGGGCCCACGACAACCTCTCCAAAGATGACAGCAATCGCAC11.6129032258064515No Hit
GGCGTATTAAACCACAGAAATCAAAACATAATATCCCAAAACCAGAAAAC11.6129032258064515No Hit
GCATAACAAAGCATAAAAAAACAACCCAGACCAAGTGTATCGAAAAAACT11.6129032258064515No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)