FastQCFastQC Report
Fri 18 Mar 2022
HJKC5AFX3_n01_T12_Rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKC5AFX3_n01_T12_Rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT24.25531914893617No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGATTAATGTCGATTAGT24.25531914893617No Hit
AGACCCGATCACTTGTGGAAAGGACGAAACACCGATTGATGTCGATTAGT12.127659574468085No Hit
AAAACAGAAAAATACAAAGTTAAATAACTCAACAGTGGCCTACTCAATTA12.127659574468085No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGATTGATGGCGATTAGT12.127659574468085No Hit
AGATTCAAAAACCACACCAAAACCAAAAAAAAACAAAAAAACCAATAACA12.127659574468085No Hit
CCGGACTTGGCGGGGGTGGAGGTGTGGATGTAGGGGAAATTGGGTTTGTT12.127659574468085No Hit
GGGTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12.127659574468085No Hit
GGTCGCGGACGAGGATCAACCCTTTTCTGCCTTTCTTCCTCCTCATAGAA12.127659574468085No Hit
GAACTAGAGTCTTGTGGAAAGGACGAAACACCGTGAAGCTGTGCCTTAAA12.127659574468085No Hit
TTTCATATTGGACTTCTATAAACTGTAAAACTGATTAGGGCGATATGGGT12.127659574468085No Hit
AAAACAAAATAAAAAAAAAAAAAAAAATGAAAAAGGCAAAAAACAACCAA12.127659574468085No Hit
ATCTAAAAAACATAAAAATAAATAAAATCAATAGACACTCTAGAACAAAC12.127659574468085No Hit
TCGCAAAATACAAACTCACCAAAATAAACCCAACCTAATTAAGAAGCTTA12.127659574468085No Hit
GCAGAAAGGCAACAAAAGCTCATTTCAACACCTATTAACAGGCAATTGTG12.127659574468085No Hit
TGGATAATTTTCGCGGAGATACAAAGTCCCAACCATAATAAGGTTTCATA12.127659574468085No Hit
AAAAGTTATAAAAAAACAATAAACATAACCACAGATAAAAAAGTAATAAG12.127659574468085No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTTGATGTCGATTAGTA12.127659574468085No Hit
ATTTTAAACCCAAAAAAAAATCTCCAAATTGGGCAAAAGGACTCTTTCGC12.127659574468085No Hit
GGGCTGGGGGGGTGGGGGGTGTTGTGGGTGGTTGTGGTTGTTTGGTTGTT12.127659574468085No Hit
GGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGG12.127659574468085No Hit
CAAAGACAAAAAATATACCATAAAATACAGCCAAAAACGAACAGAAACGT12.127659574468085No Hit
GGGGTTGCCCCCGCGAAGCGCGGTAGGTTTTCTGCTTAGGAGTTTAATCA12.127659574468085No Hit
TGCCTGCGAGGTTGAAATTCCATTGGTCGAATTGGTACTAATACAATTCC12.127659574468085No Hit
ATACACGCGCTCTTGTGGAAAGGACGAAACACCGATTGATGTCGATTAGT12.127659574468085No Hit
TAAATCACGGCTAAAGAAATCAAACTGATCGTCAAACTACAAAAATTGAA12.127659574468085No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGCTGAGTCCTGAAACAT12.127659574468085No Hit
AAAGCAAGGTGATACAAAAATCAAGATAATAGGAGACAAACACAATGCGC12.127659574468085No Hit
GGGGGTGTGTTGATTATTGGTAGGTCTTGGCATGGAGTATTAGGCTATGT12.127659574468085No Hit
AGACCCGATCTCTTGTGGAAAGGACGAAACACCGATTGATGTCGATTAGT12.127659574468085No Hit
TGGCCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12.127659574468085No Hit
AAATACGAGGAACACTGCAAATAAATTATGCCAAACTAAAAGACACTAAC12.127659574468085No Hit
GGTAATGGGGTGGTTTGGGGTTGGGGGTGTGTTTGTGTTTGTGTGTTTTT12.127659574468085No Hit
GTACGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12.127659574468085No Hit
CTCGGTAACAGGGAGCGGTACCACTAGACGTAGAGAAAAGGAAAAAAACA12.127659574468085No Hit
GAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT12.127659574468085No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGGACGGACAGGGTCGAA12.127659574468085No Hit
TTTGTCGATCTCTTGTGGAAAGGACGAAACACCGGCTAGTGGAAATACCT12.127659574468085No Hit
AAAGTCACCCGTATAATAGCCACTTAACTAAGACTGACATAACCCAGCTA12.127659574468085No Hit
ATTAAGGCAATGCTGCAAAGCCCTTATAACCCAGTAGTAAGAATATTATG12.127659574468085No Hit
ACAGCAACCAGCCTCAAACGTCGACAAATCCCTCAAAAACACAAAATATA12.127659574468085No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTGTGTTGTGGGGCGTG12.127659574468085No Hit
GATTGGGGATTGAGAAAGAGTAGAAATGCCACAAGCCTCAATAGCAGGTT12.127659574468085No Hit
AAAGATAAAAACAAAAAAAAAAATAAAACAATATAAAAACCAAACAAACA12.127659574468085No Hit
GAAATAAGAAACGAAAATTCAAAGCGTAAAGAAAAGCAAAGGAGGAAAGC12.127659574468085No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)