FastQCFastQC Report
Fri 18 Mar 2022
HJKC5AFX3_n01_T12_Rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKC5AFX3_n01_T12_Rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46.896551724137931No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAGCCAGAGATACTGGGC23.4482758620689653No Hit
TGGGGGAAAACGAACAAGCGCAAGAGTAAACATAGTGCCATGCTCAGGAA11.7241379310344827No Hit
GGCGCAAAAACAAGATAGAATAAAGAAAAATAAAATAAAATAAAAAAAAG11.7241379310344827No Hit
AACCAACTGATCTACACAAATAACAACCAAGCCCAAACGAGCATGATAAA11.7241379310344827No Hit
CCACCGATTCTAAAAAAACAAATAAATAACCAAAAGCCGAAAACACACAT11.7241379310344827No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCTATTTATGAAAGAGGC11.7241379310344827No Hit
GGGAACCTGTACCGCACGATAAAACTCTAACGCACGATTCCTTCTTCAAT11.7241379310344827No Hit
TGGCCCGGGATCAACCCCGCGTGGCCAGCCTGAGCAGGCGTTCTCTCTTG11.7241379310344827No Hit
GTTTAGGGGTGGGGGGTGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGG11.7241379310344827No Hit
CGGCAAGAACCGGCGCGGTGGGGGGGGCGTTTGTAACTAGGGCGCTAAAG11.7241379310344827No Hit
GGGGTAAAACAGGGGGGGAAAAACAACACCAACAATAACACGTCAAAAAG11.7241379310344827No Hit
CAGGCCAAACAGGATGATGTAAAAGACAGACAAAGTAAAAAAAGTAAAAC11.7241379310344827No Hit
GGGGAGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.7241379310344827No Hit
GGCGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.7241379310344827No Hit
GGGGTTTCGCCGACGCGGTATAAGAAAAAATGTTAAGGTAACAAGGCATA11.7241379310344827No Hit
GAAACATGATTAAACTCCTAAGCAGAAAACCTACCGCGCTTCGCTTGGTC11.7241379310344827No Hit
GTATAGGGGGGGGGGGGGGGGGGGGGGTGGGGGTGGGGGGGGGGGGGGGG11.7241379310344827No Hit
GGGCAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.7241379310344827No Hit
GGGCTATTAGGGCTTGTCAAACGGGCGAAGTAGGATGACGGGATGAACCT11.7241379310344827No Hit
ACGATAGAATGCAAACACTGAAGATGATGAAAAATACCCCTCGAACAACC11.7241379310344827No Hit
AATAACAAACCTAAAACACAAATACAAAAGAATATACTATCACAAAACTT11.7241379310344827No Hit
GGGGGCGGTTCGGGGGCTTCAATGCGGACGTCTATCGGTATGCTGCAAGC11.7241379310344827No Hit
GGGTCAGAAAACAGAACAACAACCGCACAAACCACTAACAAGACTAACCC11.7241379310344827No Hit
AAACTAACAGAAAAAAGAAAACAAGGAAACTACACAGCCACAACATATCA11.7241379310344827No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAAAGGAACGACACACA11.7241379310344827No Hit
AAAAATGACAAAACTAAATACAACATATATAAAAAAAACAATGAGAAAAT11.7241379310344827No Hit
TGGAGTCCGGCAACACAAGGTTCCATTCCAGACTAGGTGGAATCACGTGC11.7241379310344827No Hit
GAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACTGTGAGGAAGTGGA11.7241379310344827No Hit
GGGGGGCGGTTCCTTATTTGTGGGGCTTACGTGTTGTTGTGAGTTGCGCG11.7241379310344827No Hit
GGGGGGCCGGCGGCGGGGCCCGCGCCGGGCCGGCGGCCGGGCCCGGCGGG11.7241379310344827No Hit
GAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTCA11.7241379310344827No Hit
GGGCATGTAAAACAGGAGATAAAGAGGAACAATAAACAATGTACGAAAAA11.7241379310344827No Hit
GCTGCGGGGGGGGGGTGTGGGGGGGGGGGGGGGGGTTGGGGGGGGTGGGG11.7241379310344827No Hit
TCCCAACGATAAAAACTCAAGACACACAAACAAACATCAAAAACAAACAT11.7241379310344827No Hit
GGAGTGGGGGTGTTGGGGGGGGTGTGGGTGTGTGTGTTTGTTTTTGTGTT11.7241379310344827No Hit
TGTGGTGAAACGCGGATGAAAAAAACCGAGGAAACAACAAAATCAATACT11.7241379310344827No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGATAGGGCCATCTGAGG11.7241379310344827No Hit
GGGGGTAGCTGTATTAGTGTGAGGTCTGAGATGGGTGTGTGAAGTAATCG11.7241379310344827No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTCAATTAGAAGACAAGA11.7241379310344827No Hit
ATATGAAAACGGTGTAAGGATCCTGGGCAAAACAAAAACGGTAAACCCCG11.7241379310344827No Hit
AAAANGGAAAAAAAAGCAATGAAAAAAAGNGCNTCAAATNATAACGCNAA11.7241379310344827No Hit
AAAAGAAAAAAAAAAAAAGGAACAAAACTAAAAACAGACAAACAAAAAAA11.7241379310344827No Hit
GGGGGTCTTTTCATTCGTGGTCTTTATTGGTATTGGTTGGTAGTAAATTG11.7241379310344827No Hit
TTGTGAAATTAATATTTAGTAAGCGAACAATATGAATTTGTCCGTATAGT11.7241379310344827No Hit
GGGGTGTGTTATAAAAAAATGATTTACCAGAATTTGAATGAGAGTGTTAG11.7241379310344827No Hit
CAATTAGCGACAAAAAAATGCAAACCAGCACAAGTCCAAAAAGACAACAA11.7241379310344827No Hit
CTTTACAGGAAAAAAAAACAGGAAAGAGAGGGCTAGCTCGCCACTGGACA11.7241379310344827No Hit
GGGTGATCTAAAACATTAGAATTAGTGTTACAAAAATAACAATTACGAAA11.7241379310344827No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTAAGTAGATCAATGAA11.7241379310344827No Hit
CTAATGAGGCTTGTGGCATTTCTACTCTTTCTCAATCCCCAATGCTTGGC11.7241379310344827No Hit
AGCGCAGCCAACCAGCCGCCAAATAAAACGCAAAGATCCAAAGATGCAGC11.7241379310344827No Hit
CGGGAAGAGAAGGGGGGGTAATGACCGGAAACAAGGCAATTATACAAAAC11.7241379310344827No Hit
GGGGTGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.7241379310344827No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)