FastQCFastQC Report
Fri 18 Mar 2022
HJKC5AFX3_n01_T0_Rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKC5AFX3_n01_T0_Rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGGGGGGTGGGGGGTGTGTGGTGGTTTTTGTTGTGTGGGTTTTGTGG11.694915254237288No Hit
GAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGTACTTCGTGATGGAGA11.694915254237288No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCAACCGCGTGTCAGTCA11.694915254237288No Hit
GGCCGAATCATTATAGAGGTATTCATAACATGTATCAAATAAAAAAAAAT11.694915254237288No Hit
CAAAGCCTGCAAAAATCAGCGGAAAAATAAGGATCACCAACACACCGGTA11.694915254237288No Hit
GTAAGAGACATAAAAATAGAGCATGAAGATCTGAATGAATAAGGAAGAGA11.694915254237288No Hit
ACGTCCAAAACACAAACAACAAAAAAACTGGAAAATCAAACCCAAAAAAA11.694915254237288No Hit
ACCGTCCGGTAAAACACGAAGGGCTTAGATGGGTAACGGCAGCAGGGGTG11.694915254237288No Hit
TCTGAACTTAAGCAAATCGATTATGGGGTCTTAAGTTTGTTTGTTGCATT11.694915254237288No Hit
CGGGTCGAAAAAAAATAGTTAAAAAAACTGAACATAGTAACTACAAAAAA11.694915254237288No Hit
CCCATTATTCCAAAAAAAGAGCAAAAAGAAAAAAAAAACCAACATAACAT11.694915254237288No Hit
CCACACACCTTAGAACAAAAAAAAAAAAAAATAAAATTCTCAAAAAAAAA11.694915254237288No Hit
GCCCAGAAAAAAAAAAAATGAAAAAAAAAGAAATAACAAAACTCGAACAC11.694915254237288No Hit
GCAACAATAAATATTGTATTGTTTAATGAATTTGTAATTATAAACTATTA11.694915254237288No Hit
GGAAAAAACATGCAAAATAAAAAAAGCAAATCAGCCTAAAAGAGTATAAC11.694915254237288No Hit
GCGTAATGACTACACAAAATTGGGAAAATAACGAACGCGGATTTAGGGGT11.694915254237288No Hit
CAGAGAACTAAGTCCTGAGGACAATAAAACAAACACACCTATCGAACTTT11.694915254237288No Hit
GAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTGGAGTTAAGAGAGTCC11.694915254237288No Hit
CGATCTTGATCGTCTTGTGGAATGGACGAAACACCGCACTCAGCTCCTGG11.694915254237288No Hit
AGCCCTAGCCAATCCAACATCAAACTCAGACCAAAAAATAAATAAGACAA11.694915254237288No Hit
GGGGGAACGGGGGGGGGGATTCGGGGTGTACAGTGTTTAAGGATCCGAGC11.694915254237288No Hit
TGTCGAACATAAAATTTAGTAAACAGACAACAAGAGATAAAAGAAAAACA11.694915254237288No Hit
TGTCGATCTCCGGAAGAATCTATCATGGCTGGTTCAGTAAGTTAGCCAAT11.694915254237288No Hit
GGGATGTATCCGTGTTTGCGAATGATTATAAAAACGTACAGGGGAAAAAT11.694915254237288No Hit
TGGAACCAATGGGGCAAGACCTATTTGCAATCAATCACATGTAGAATAGA11.694915254237288No Hit
GGGTCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.694915254237288No Hit
GGAGTAAGGGCGGCGAGGGTGTAACAAGTTAGAAAATAGACTGAAGGAAA11.694915254237288No Hit
ATCGTCAACGTTATATTTTGATAGTTTGACGGTTAATGCTGGTAATGGTG11.694915254237288No Hit
GCATCTAAGCACAACAAGAATATTAAACAAATAAATGTCATTAACGCATT11.694915254237288No Hit
GGTGGGCGTTAAAATGATGTGCGGACTGGTAGTTCAAAAAGGGTCACTAA11.694915254237288No Hit
GGGTTGGGTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.694915254237288No Hit
GCCGATAGGAGCTCCGGGATTATGAGGATAGAAAACCAAAACGAATAAAA11.694915254237288No Hit
ATTGCGTCGTGGCCTTGCTATTGACTCTACTGTAGACATTTTTACTTTTT11.694915254237288No Hit
CGGATAAAAGGAAGGCAAAGACAGGAAAAAAAGTGCTAAGTGTGATATGA11.694915254237288No Hit
GGGGGTAATTGATCAAAAAATACAGCAAAAACCAATGCCGAATACGTATA11.694915254237288No Hit
GTCGGACTTTTGCCATGAGCGTTTTCATGATATACATCTTCTTAAGTTTG11.694915254237288No Hit
GCCGGACATGCTTGTAAAGGAATACATTAATAAACAATAACGATACAAAA11.694915254237288No Hit
CGAGAGAAAGCGCCAAACAAACGACGATTCAATAGGACATCACTTCTTGT11.694915254237288No Hit
ACCGGACCTTAGCAACAACGCCATCTGCGCAATCACACTTCAGGCTCAAT11.694915254237288No Hit
GTGGTGAGATGAAGAATAAGTTCGGTAACGATCGAATCAATCTCTATTGA11.694915254237288No Hit
AAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAGTGCCAAGAGGACTTG11.694915254237288No Hit
AGCAAGCGAAGGAGGGCAAGGAAAATAAGGGAAATTACAAAAATGTGAAA11.694915254237288No Hit
GAAGTAGAGTCTTGTGGAAAGGACGAAACACCAACAAGAACAGCCAATGG11.694915254237288No Hit
GATGAGTCGTCTACATAATATAGTAGTTCAGCAAAAGGTCATAAGGTGTA11.694915254237288No Hit
GTTCAAAACCAATGAACCAAAAGAACTACGGATAGCGGAAGAATGCCTAT11.694915254237288No Hit
GGGGGAGAATATATCAAACAAAAAATTAAATTAGAATTATAAAAGACAAA11.694915254237288No Hit
ATGGTCGTGATATACCCTTACACAACATAATAACAATAACAATAAGATAA11.694915254237288No Hit
TTTGGGTCAGTGTGGTGAATCGAGCAACAAATATCCGTATGTGACAACGC11.694915254237288No Hit
GGGGGTAAAGATTCAATGCTAAAATCGACCCAAACGTAAACAACAAAAAC11.694915254237288No Hit
CTTTTGAAGAAATAACATCATGGTAACGCTGCATGAAGTAATCACGTTCT11.694915254237288No Hit
AACGTGTCAAAAAAAAACACCAAAAGACAATACGACAAAAAACAAAAAAG11.694915254237288No Hit
AAAACACAAGTTACAAAAAGAAAAACAATTCATGAAAAAGACGACAAAAA11.694915254237288No Hit
GGTTCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.694915254237288No Hit
CGCTGGTATTTGGGTACCTGTGGTTTATCGTTTTGAAAATGAGAATTTCT11.694915254237288No Hit
GGGGTGGGCGGGGGGGGCAGCGGGGTGTGGGCTGGCGGGGGCTGGCGGGG11.694915254237288No Hit
GATGGAAAAGATTAAAACGTACAACTATCGCCCTTCACCATAGAATAAAA11.694915254237288No Hit
AGTGCAAATACAGGCAAGAAAATGACATAAACATGAAAAAAAGAAAAAAA11.694915254237288No Hit
GTAATCATAGCAAGAAGAAAAAAGATGCAATCTCCGAGGCTAAGAAACCG11.694915254237288No Hit
CGTACAGATTGCACAAACCCCCCGGTACAAAAGAAAAAAGGCACTAAAGC11.694915254237288No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)