FastQCFastQC Report
Fri 18 Mar 2022
HJKC5AFX3_n01_T0_Rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKC5AFX3_n01_T0_Rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTTGCATTTCTCAGAGGG23.7735849056603774No Hit
AGCTAAAAAATGGATCAAAAAAAGGACTAAATCGTGAAACACAAAAACCG11.8867924528301887No Hit
GGGGTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.8867924528301887No Hit
GCCTTTTATTTCTCGCCATAATTCAAACTTTTTTTCTGATAAGCTGGTTC11.8867924528301887No Hit
GGCAAACGCAAAAAAAACATTCACCATACTAACACTCTCACACACGTTAA11.8867924528301887No Hit
GTGGGTATCTAACAGCATCTACAAAAGCCCAAATCGCCTAATTCAGCCAG11.8867924528301887No Hit
TATTTGACTATAAAAAAAATAAAAAAAAGAGAACAAAATATTGAACAAAA11.8867924528301887No Hit
AAACAAAACACAGCATAACACAAACCAAATCGACATCGAAAACCCAACAC11.8867924528301887No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTGTTTACAGGAGCAATG11.8867924528301887No Hit
GACGAGTCAAAAAGCCGAAGCAAAAAGACAAACCTAGAAATGCGCAATGG11.8867924528301887No Hit
TCGTGAACCAAGCGCTAAAACATGATGATATCCCTCTTATCGTAAACTAC11.8867924528301887No Hit
TAAAAATGTTAAACAAACCAGATACAACGATAGTAGAAGAAAGTATTCAA11.8867924528301887No Hit
GGGGGTGTGGTGGGGGGGGGTGGGGGGGGGGGGGGGTGTGGGGGTGGGGG11.8867924528301887No Hit
GGGGCTGAGTATAAAGAGACGAAAAACCAGATACAGAACAAAAATGAAAT11.8867924528301887No Hit
GGCCACCCTCAGTGGCGCAGCTCGGCAACAACTAACACCAAGCCCAATAC11.8867924528301887No Hit
AAAATAAAAACAAACCCAATACAAACAACAAAAACTACACAAAACAAAAA11.8867924528301887No Hit
ATTGGTACCCCAAAAAAATCGACACTTAAAACATAGAAAAATATACTAAC11.8867924528301887No Hit
TAAAAAATAAAAAAAAATGGATTTTAAGGACGTACGAATAATGGACTTAA11.8867924528301887No Hit
GGGGGTGTGGGGGGGGGGGGGGGTGGGGTGGGGGGGGGGTGGGGGGGGGG11.8867924528301887No Hit
GTCGCAAGCGCAACAAAATAACAAAAAAAACTCAAATGGTGTACCGGGAA11.8867924528301887No Hit
TGTTATGTACGACCATGTTACTAGCTCACACCATCCTGACATAGCAAAGC11.8867924528301887No Hit
GTGTACAAATATAACACATGAAAAAACGGCTACTATTGAAAACTATCACC11.8867924528301887No Hit
TTCACTGATAAAATATACCCAAAAAAATACAAAGAGAAAAAAAAAACAAA11.8867924528301887No Hit
GGGGGATAATACGACGGTAATCACTCCCGTGAAACTATATTAAAATCTCA11.8867924528301887No Hit
TTACACGATCTCTTGTGGAAAGGACGAAACACCGTTTACAGGCTGGCTGG11.8867924528301887No Hit
AGATACAAAAAAAGTATTATAAATAAACTTGAAAGAGATAAAATATCTAA11.8867924528301887No Hit
AGCGCAAAGTCGCATGAAAGAAAAATGACTAAAACTACAAATAATACGAA11.8867924528301887No Hit
GATCGTCGGTTTTATTTTGAGGTTTTATACGGTGAAACCTAATTGGGTTA11.8867924528301887No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGGTGATAATTTCAGTGC11.8867924528301887No Hit
GTAACGTCGAACAAGATAAACTCACTAATAAGAGCATTTAAAAAATCCAA11.8867924528301887No Hit
ATCGGAAATCTAATAAAGCAGACCAGGCTCAACTATCAAAAATGAACGAC11.8867924528301887No Hit
AAATTTATAAAAAAAAAAGGACTTTTAAGCAAATTATTAAAAGAATCAAA11.8867924528301887No Hit
GGGACGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.8867924528301887No Hit
CGCCTATGCGAGACAAACCCGGGTGTAGATATAAAGAGCGCACTAGCAAT11.8867924528301887No Hit
TTTTATGATCAAAGAGTTCATATAAAACAAAAATTTTGAAAAATGATCTA11.8867924528301887No Hit
ACTACAAAATAAAAAAAAAAAAACAAAAAGAAAAGAAAAAAAAGCAACTA11.8867924528301887No Hit
GGCGGTAACCCAAACCACAACATCACAGAAATACAGACTTCTCCAAGTCA11.8867924528301887No Hit
TGTACCAGAACAAAAAAGAAAAAAAAAAATTGTAAACGGGTATAGGGGTA11.8867924528301887No Hit
GAAAGGAATAAAAAAGAACCAACAAAATACTAACATCTTACAACAAAAAA11.8867924528301887No Hit
CGCTACACGTCAATATAAACCAAAAAAATACCTAACAGCTAAAACGTGAA11.8867924528301887No Hit
GGGCGAAGAACCAAAACCAACAATTAAAAACCAAAATTAAAAAAACGAAA11.8867924528301887No Hit
GAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAGGCAGGTGGATGAGAG11.8867924528301887No Hit
GGGGTGGGGCGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.8867924528301887No Hit
AATAAACTATATAATTTACAATTTAAACCACGCCAAACAAAGAACCGAAT11.8867924528301887No Hit
TGAACCAAGTTCTAAACACATTACTGTACCACTGCGAAGAACACATAAGT11.8867924528301887No Hit
ATCTGAAAAAATAATTTAAACTGAAAAGGTAAAATGTTAAAAACCAAACC11.8867924528301887No Hit
GAACTAGAGTCTTGTGGAAAGGACGAAACACCACCGATCTGTGAGTTTAA11.8867924528301887No Hit
CATTGTCCCCAACAATATATTACAATAAACACCTAAAAAATGACAACGAG11.8867924528301887No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCAGTAACATGGGTAAGC11.8867924528301887No Hit
GCCTAAAAAATAGACTGAAAAAATTATACAAAAAATTTTTAAAAAAGAAA11.8867924528301887No Hit
ATCAAACAAAAAAAGGGATCAAAAAAACAACACCAAAGAATAACAAAAAA11.8867924528301887No Hit
TATTTGCTAAAAAAACAAGATCCATAACGATCAGGGAAATAATAACACTA11.8867924528301887No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)